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New rice resistance genes via targeted genome editing

Baufumé S., Meynard Donaldo, Reschke Maik, Solé Montserrat, Cunnac Sébastien, Szurek Boris, Guiderdoni Emmanuel, Boch Jen, Koebnik Ralf. 2013. New rice resistance genes via targeted genome editing. Phytopathology, 103 (6), suppl. S2 : S2.13. APS-MSA Conference, Austin, États-Unis, 10 August 2013/14 August 2013.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Rice is one of the most important food crop in the world providing the principal source of calories for nearly half of its population. Bacterial Leaf Blight (BLB) of rice, caused by Xanthomonas oryzae pv. oryzae, depends on direct induction of host susceptibility genes by bacterial TAL (Transcription Activator-Like) effectors. So far, efficient control of this disease relies on the use of resistant varieties. Since bacterial ability to induce specific host susceptibility genes is critical for disease development, we wish to create mutations in rice susceptibility gene promoters using the TALEN (TAL Effectore Nuclease) technology, in order to make them unresponsive to bacterial infection. We show that (i) designer TALENs cleave DNA target sites in a predictable manner, (ii) engineered bacteria secrete TALENs into culture supernatants, and (iii) engineered TALEs injected into rice embryogenic tissues are able to induce their target genes efficiently. Based on the DNA recognition code, designer TALENs were created to induce site-specific protective mutations and thus mimic the natural evolution of resistance genes on a much faster scale. With this approach, we hope to provide breeders with new strategies to generate broad and durable resistance to bacterial infections, thus reducing chemical use, providing yield stability, and increasing profitability of rice farming, in particular for smallholder farmers. (Texte intégral)

Mots-clés Agrovoc : Oryza sativa, Résistance aux maladies, Méthode de lutte, Mutation provoquée, Génie génétique, Contrôle de maladies, Xanthomonas oryzae, Gène, mécanisme de défense

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Baufumé S., IRD (FRA)
  • Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
  • Reschke Maik, CIRAD-BIOS-UMR AGAP (FRA)
  • Solé Montserrat, Martin Luther University (DEU)
  • Cunnac Sébastien, IRD (FRA)
  • Szurek Boris, IRD (FRA)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Boch Jen, Martin Luther University (DEU)
  • Koebnik Ralf, IRD (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587406/)

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