Characterization of recombinant Trypanosoma brucei gambiense translationally controlled tumor protein (rTbgTCTP) and its interaction with Glossina midgut bacteria

Bossard Géraldine, Bartoli Manon, Fardeau Marie-Laure, Holzmuller Philippe, Ollivier Bernard, Geiger Anne. 2017. Characterization of recombinant Trypanosoma brucei gambiense translationally controlled tumor protein (rTbgTCTP) and its interaction with Glossina midgut bacteria. Gut Microbes, 8 (5) : 413-427.

Résumé : In humans, sleeping sickness (i.e. Human African Trypanosomiasis) is caused by the protozoan parasites Trypanosoma brucei gambiense (Tbg) in West and Central Africa, and T. b. rhodesiense in East Africa. We previously showed in vitro that Tbg is able to excrete/secrete a large number of proteins, including Translationally Controlled Tumor Protein (TCTP). Moreover, the tctp gene was described previously to be expressed in Tbg-infected flies. Aside from its involvement in diverse cellular processes, we have investigated a possible alternative role within the interactions occurring between the trypanosome parasite, its tsetse fly vector, and the associated midgut bacteria. In this context, the Tbg tctp gene was synthesized and cloned into the baculovirus vector pAcGHLT-A, and the corresponding protein was produced using the baculovirus Spodoptera frugicola (strain 9) / insect cell system. The purified recombinant protein rTbgTCTP was incubated together with bacteria isolated from the gut of tsetse flies, and was shown to bind to 24 out of the 39 tested bacteria strains belonging to several genera. Furthermore, it was shown to affect the growth of the majority of these bacteria, especially when cultivated under microaerobiosis and anaerobiosis. Finally, we discuss the potential for TCTP to modulate the fly microbiome composition toward favoring trypanosome survival.

Mots-clés libres : Bacteria midgut flora, Glossina, Trypanosoma brucei gambiense, Translationally controlled tumour protein (TCTP)

Classification Agris : L73 - Maladies des animaux
000 - Autres thèmes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Bossard Géraldine, CIRAD-BIOS-UMR INTERTRYP (FRA)
• Bartoli Manon, IRD (FRA)
• Fardeau Marie-Laure, IRD (FRA)
• Holzmuller Philippe, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-8919-9081
• Ollivier Bernard, IRD (FRA)
• Geiger Anne, IRD (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587509/)

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