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MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees

Ben Amira Maroua, Mom Robin, Lopez David, Chaar Hatem, Khouaja Ali, Pujade-Renaud Valérie, Fumanal Boris, Gousset-Dupont Aurélie, Bronner Gisèle, Label Philippe, Julien Jean-Louis, Triki Ali Mohamed, Auguin Daniel, Venisse Jean-Stéphane. 2018. MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees. PloS One, 13 (3):e0193760, 23 p.

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Url - jeu de données : https://figshare.com/articles/MIP_diversity_from_i_Trichoderma_i_Structural_considerations_and_transcriptional_modulation_during_mycoparasitic_association_with_i_Fusarium_solani_i_olive_trees/5988817

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Abstract : Major intrinsic proteins (MIP) are characterized by a transmembrane pore-type architecture that facilitates transport across biomembranes of water and a variety of low molecular weight solutes. They are found in all parts of life, with remarkable protein diversity. Very little is known about MIP from fungi. And yet, it can legitimately be stated that MIP are pivotal molecular components in the privileged relationships fungi enjoy with plants or soil fauna in various environments. To date, MIP have never been studied in a mycoparasitism situation. In this study, the diversity, expression and functional prediction of MIP from the genus Trichoderma were investigated. Trichoderma spp. genomes have at least seven aquaporin genes. Based on a phylogenetic analysis of the translated sequences, members were assigned to the AQP, AQGP and XIP subfamilies. In in vitro and in planta assays with T. harzianum strain Ths97, expression analyses showed that four genes were constitutively expressed. In a mycoparasitic context with Fusarium solani, the causative agent of fusarium dieback on olive tree roots, these genes were up-regulated. This response is of particular interest in analyzing the MIP promoter cis-regulatory motifs, most of which are involved in various carbon and nitrogen metabolisms. Structural analyses provide new insights into the possible role of structural checkpoints by which these members transport water, H2O2, glycerol and, more generally, linear polyols across the membranes. Taken together, these results provide the first evidence that MIP may play a key role in Trichoderma mycoparasitism lifestyle.

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Ben Amira Maroua, INRA (FRA)
  • Mom Robin, Université de Clermont-Ferrand (FRA)
  • Lopez David, Université de Clermont-Ferrand (FRA)
  • Chaar Hatem, INAT (TUN)
  • Khouaja Ali, INAT (TUN)
  • Pujade-Renaud Valérie, CIRAD-BIOS-UMR AGAP (FRA)
  • Fumanal Boris, Université de Clermont-Ferrand (FRA)
  • Gousset-Dupont Aurélie, Université de Clermont-Ferrand (FRA)
  • Bronner Gisèle, Université de Clermont-Ferrand (FRA)
  • Label Philippe, Université de Clermont-Ferrand (FRA)
  • Julien Jean-Louis, Université de Clermont-Ferrand (FRA)
  • Triki Ali Mohamed, Institut de l'olivier (TUN)
  • Auguin Daniel, Université d'Orléans (FRA) - auteur correspondant
  • Venisse Jean-Stéphane, Université d'Orléans (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587687/)

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