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Genomic structure, QTL mapping, and molecular markers of lipase genes responsible for palm oil acidity in the oil palm (Elaeis guineensis Jacq.)

Domonhedo Hubert, Cuellar Térésa, Espéout Sandra, Droc Gaëtan, Summo Marilyne, Rivallan Ronan, Cros David, Nouy Bruno, Omoré Alphonse, Nodichao Leifi, Arondel Vincent, Ahanhanzo Corneille, Billotte Norbert. 2018. Genomic structure, QTL mapping, and molecular markers of lipase genes responsible for palm oil acidity in the oil palm (Elaeis guineensis Jacq.). Tree Genetics and Genomes, 14 (5):69, 12 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q2, Sujet : FORESTRY / Quartile : Q3, Sujet : GENETICS & HEREDITY

Abstract : The degradation of triglycerides in oil palm fruit due to an endogenous lipase in the pulp is the main reason for acidification of palm oil, which causes major economic losses and is currently mainly associated with the FLL1 gene. We designed this study to identify all the major genes controlling differences in acidity and lipase activity in the oil palm fruit mesocarp and determine a molecular markers kit to allow marker-assisted selection of commercial varieties with low acidity. Not only one gene (FLL1) but three closely linked genes including FLL1 were found and characterized in LM2T_EgCIR184O12c, a bacterial artificial chromosome sequence of 231 kb. Intra-gene PCR-based markers were designed for these genes. A QTL gene co-localization analysis for oil acidity (percentage of fatty acids released) was performed on two mapping populations. It evidenced a single major QTL at our lipase gene loci, explaining 84 to 92% of phenotypic variation, and validating the main genetic control of palm oil acidification by FLL1 and/or by the two new lipase genes. The three lipase genes had high homology to demonstrated triacylglycerol lipases. While FLL1 shows the highest expression levels, the two other genes may also contribute to oil acidity. Our molecular markers of lipase genes and the associated major QTL is an important step towards marker-assisted selection of commercial varieties with low acidity.

Mots-clés Agrovoc : Huile de palme, Elaeis guineensis, Qualité, Acidité, Triglycéride, Triacylglycérol lipase, Marqueur génétique, Locus des caractères quantitatifs

Mots-clés géographiques Agrovoc : Bénin, Côte d'Ivoire

Mots-clés libres : QTL, Lipases, Palmier à huile, Qualité de l'huile, Oil quality, Oil palm

Classification Agris : Q04 - Food composition
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Domonhedo Hubert, INRAB (BEN)
  • Cuellar Térésa, CIRAD-BIOS-UMR AGAP (FRA)
  • Espéout Sandra, CIRAD-BIOS-UMR AGAP (FRA)
  • Droc Gaëtan, CIRAD-BIOS-UMR AGAP (FRA)
  • Summo Marilyne, CIRAD-BIOS-UMR AGAP (FRA)
  • Rivallan Ronan, CIRAD-BIOS-UMR AGAP (FRA)
  • Cros David, CIRAD-BIOS-UMR AGAP (CMR) ORCID: 0000-0002-8601-7991
  • Nouy Bruno, PalmElit (FRA)
  • Omoré Alphonse, INRAB (BEN)
  • Nodichao Leifi, INRAB (BEN)
  • Arondel Vincent, CNRS (FRA)
  • Ahanhanzo Corneille, Université de Bordeaux (FRA)
  • Billotte Norbert, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588653/)

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