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Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra

Fu Tingting, Touboul David, Della Negra Serge, Houël Emeline, Amusant Nadine, Duplais Christophe, Fisher Gregory L., Brunelle Alain. 2018. Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra. Analytical Chemistry, 90 (12) : pp. 7535-7543.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Outlier, Sujet : CHEMISTRY, ANALYTICAL

Abstract : Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis.

Mots-clés Agrovoc : Bois, Forêt tropicale humide, Métabolite, Bois de coeur, Spectrométrie de masse, Lauraceae

Mots-clés géographiques Agrovoc : Guyane française

Mots-clés complémentaires : Sextonia rubra

Mots-clés libres : Sextonia rubra, Mass spectrometry imaging, Heartwood formation, Bioactives metabolites, Amazonian wood species

Classification Agris : K50 - Processing of forest products
F60 - Plant physiology and biochemistry
U30 - Research methods

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

Auteurs et affiliations

  • Fu Tingting, CNRS (FRA)
  • Touboul David, CNRS (FRA)
  • Della Negra Serge, CNRS (FRA)
  • Houël Emeline, CNRS (FRA)
  • Amusant Nadine, CIRAD-ES-UMR Ecofog (GUF)
  • Duplais Christophe, CNRS (FRA)
  • Fisher Gregory L., Physical Electronic (USA) - auteur correspondant
  • Brunelle Alain, CNRS (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588713/)

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