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An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae)

Virgilio Massimiliano, Daneel J.H., Manrakhan Aruna, Delatte Hélène, Meganck Kenny, De Meyer Marc. 2019. An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae). Bulletin of Entomological Research, 109 (3) : pp. 376-382.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : ENTOMOLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : The Ceratitis FAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (Ceratitis anonae, Ceratitis fasciventris, Ceratitis quilicii, Ceratitis rosa) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (C. rosa, C. quilicii), we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first tested in silico and then used for the actual genotyping of C. quilicii and C. rosa, resulting in the correct identification of all male reference specimens. Here, we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and female C. anonae, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult female C. fasciventris/C. quilicii/C. rosa and (c) molecular identification via a reduced panel of six microsatellite markers for samples including only C. quilicii and C. rosa. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identify species within the FAR complex for their early detection and monitoring.

Mots-clés géographiques Agrovoc : Botswana, Kenya, Réunion, Malawi, Mozambique, Maurice, Afrique du Sud, Eswatini, République-Unie de Tanzanie, Zimbabwe

Classification Agris : H10 - Pests of plants
L60 - Animal taxonomy and geography

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agence(s) de financement européenne(s) : European Commission

Programme de financement européen : FP7

Agence de financement hors UE : Federaal Wetenschapsbeleid

Auteurs et affiliations

  • Virgilio Massimiliano, Musée royal de l'Afrique centrale (BEL) - auteur correspondant
  • Daneel J.H., Citrus Research International (ZAF)
  • Manrakhan Aruna, Citrus Research International (ZAF)
  • Delatte Hélène, CIRAD-BIOS-UMR PVBMT (REU)
  • Meganck Kenny, Royal Museum for Central Africa (BEL)
  • De Meyer Marc, Musée royal de l'Afrique centrale (BEL)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588814/)

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