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Integrated sequences of Banana streak virus (BSV) in banana plantain: how does gene silencing-based plant defense system work?

Ricciuti Emeline, Massé Delphine, Hoestachy Bruno, Iskra Caruana Marie-Line. 2018. Integrated sequences of Banana streak virus (BSV) in banana plantain: how does gene silencing-based plant defense system work?. . Bordeaux : s.n., Résumé, 1 p. COST iPLANTA CA15223, WG1 Meeting "Comparing siRNA and miRNA technology and role for improving perennial plants", Bordeaux, France, 17 July 2018/18 July 2018.

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Additional Information : Emeline Ricciuti est l'xpert invitée du Cirad

Abstract : The majority of cultivated bananas are intra and interspecific hybrids of the two main species Musa acuminata (denoted genome A) and Musa balbisiana (denoted genome B). The Banana streak virus (BSV) is responsible of the banana streak mosaic disease and causes severe damage on the Musa acuminata hybrids, which are extremely susceptible. The virus is mealybugs- vector transmitted from plant to plant. Curiously, BSV also exists as endogenous sequences named (eBSV) within all B genomes and can give rise active viruses leading to a systemic infection in banana plants. The banana plantain is a natural triploid interspecific hybrid AAB and harbors viral sequences for two BSV species within its B genome. It spontaneously develops BSV infections following abiotic stresses by releasing active viral genome from eBSVs. However, no epidemic has been reported so far, whereas several plantains regularly show infections in the fields. We suspected that the banana plantain regulates such endogenous viral infections by a gene silencing-based plant defense mechanism resulting from a co-evolution process. Our project aims understanding how this pathosystem works by following after an endogenous infection the kinetic of the virus distribution in the plant via molecular (IC-PCR, qPCR) and histologic (serological detection) markers as well as the small viral RNA profiles involved in gene silencing (TGS, PTGS) via Northern blots, NGS and LNA probes.

Mots-clés libres : Banana streak virus, Plantain, Integrated sequences, Gene silencing, Epidemiologic risk

Auteurs et affiliations

  • Ricciuti Emeline, CIRAD-BIOS-UMR BGPI (FRA)
  • Massé Delphine, SPV (Réunion) (REU)
  • Hoestachy Bruno, SPV (Réunion) (REU)
  • Iskra Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449

Source : Cirad-Agritrop (https://agritrop.cirad.fr/588896/)

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