Recovery of oil palm (Elaeis guineensis Jacq.) somatic embryos cryostored for 20 years

Beulé Thierry, Ilbert Pascal, Adeoti Adéola Zouri-Kifouli, Durand-Gasselin Tristan, Dumet Dominique, Engelmann Florent, Morcillo Fabienne. 2018. Recovery of oil palm (Elaeis guineensis Jacq.) somatic embryos cryostored for 20 years. CryoLetters, 39 (1) : pp. 60-66.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q4, Sujet : BIOLOGY / Quartile : Q4, Sujet : PHYSIOLOGY

Abstract : BACKGROUND: A cryopreservation protocol has been established for oil palm somatic embryos (SEs), the efficiency of which must be evaluated, both in terms of regeneration and of long-term storage capacity, before its large-scale routine use. OBJECTIVE: To test the survival and recovery of 29 clones of oil palm somatic embryos cryostored for 20 years. MATERIALS AND METHODS: Clumps of SEs were pregrown for 7 days on medium containing 0.75 M sucrose, dehydrated in air-tight containers containing silica gel to moisture contents between 19-35% fresh weight, and then immersed directly in liquid nitrogen and stored in cryotanks for 20 years. RESULTS: Survival of SEs cryopreserved and rewarmed immediately displayed an average value of 19.1% for the 29 clones tested while survival of SEs rewarmed after 20 years of cryostorage was significantly higher, with an average of 33.2% for the 28 surviving clones. Out of these 28 surviving clones, three were lost due to contamination or regrowth decline, six produced only shoots and the rest proliferated. CONCLUSION: I t is possible to cryostore oil palm SEs for extended periods and to regenerate proliferating cultures and plantlets from the cryopreserved material. The cryopreservation protocol established can thus be efficiently used to store oil palm germplasm and to manage large-scale production in industrial laboratories.

Mots-clés Agrovoc : Elaeis guineensis, Cryoconservation, Embryon somatique, Germplasm, Régénération in vitro

Mots-clés géographiques Agrovoc : Bénin, France

Mots-clés libres : Cryoconservation, Oil palm, Somatic embryos

Classification Agris : F30 - Plant genetics and breeding
U30 - Research methods

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Beulé Thierry, CIRAD-BIOS-UMR DIADE (FRA)
  • Ilbert Pascal, CIRAD-BIOS-UMR DIADE (FRA)
  • Adeoti Adéola Zouri-Kifouli, CIRAD-BIOS-UMR DIAPC (FRA)
  • Durand-Gasselin Tristan, PalmElit (FRA)
  • Dumet Dominique, IRD (FRA)
  • Engelmann Florent, IRD (FRA) - auteur correspondant
  • Morcillo Fabienne, CIRAD-BIOS-UMR DIADE (FRA)

Source : Cirad-Agritrop (

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