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Development of HP-TLC method for rapid quantification of sugars, catechins, phenolic acids and saponins to assess Yam (Dioscorea spp.) tuber flour quality

Lebot Vincent, Malapa Roger, Molisalé Tari. 2019. Development of HP-TLC method for rapid quantification of sugars, catechins, phenolic acids and saponins to assess Yam (Dioscorea spp.) tuber flour quality. Plant Genetic Resources, 17 (1) : pp. 62-72.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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development_of_hptlc_method_for_rapid_quantification_of_sugars_catechins_phenolic_acids_and_saponins_to_assess_yam_dioscorea_spp_tuber_flour_quality.pdf

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Quartile : Q4, Sujet : PLANT SCIENCES

Abstract : Eight yam species (Dioscorea spp.) represented by 522 accessions (landraces and hybrids) were analysed for the individual sugars, catechins, phenolic acids and saponins contents of their tuber flours. Maltose, sucrose, glucose and fructose were quantified. Reducing sugars mean values were highly variable within species and ranged from 0.16%dry weight (DW) (SD ± 0.12) in D. dumetorum to 3.15%DW (±2.49) in D. esculenta. Maltose was detected only in D. esculenta. Chlorogenic acid, gallic acid and other phenolic acids ranged from 4.33 mg/g DW in D. bulbifera to 4.87 mg/g in D. alata and 9.55 mg/g in D. nummularia but were not detected in other species. Catechins (epicatechin and catechin) were highest in D. bulbifera bulbils (25.18 mg/g) and tubers (6.96 mg/g), and lowest in D. esculenta (0.32 mg/g). Their content is significantly correlated with dark flour colour and they most likely contribute to the oxidation of tuber flesh. Saponins (dioscin and gracillin) were quantified in only two species: D. cayenensis (5.94 mg/g, SD ± 3.78) and D. esculenta (3.74 mg/g, SD ± 3.72). Varietal selection may tend to reduce sugars levels and increase secondary metabolites with bioactive properties. HP-TLC is a suitable technique for the rapid quantification of compounds related to yam tuber flour quality.

Mots-clés Agrovoc : Igname, Composition chimique, Sucre, Technique analytique

Mots-clés libres : Ignames, Métabolites secondaires, Analyse chimique, Diversité génétique, Sucres

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry
Q04 - Food composition

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations

  • Lebot Vincent, CIRAD-BIOS-UMR AGAP (VUT) - auteur correspondant
  • Malapa Roger, VARTC (VUT)
  • Molisalé Tari, VARTC (VUT)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589227/)

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