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Interaction between the GROWTH-REGULATING FACTOR and KNOTTED1-LIKE HOMEOBOX families of transcription factors

Kuijt Suzanne J.H., Greco Rafaella, Agalou Adamantia, Shao Jingxia, 't Hoen Corine C.J., Övernäs Elin, Osnato Michela, Curiale Serena, Meynard Donaldo, van Gulik Robert, de Faria Maraschin Simone, Atallah Mirna, De Kam Rolf J., Lamers Gerda E.M., Guiderdoni Emmanuel, Rossini Laura, Meijer Annemarie H., Ouwerkerk Pieter B.F.. 2014. Interaction between the GROWTH-REGULATING FACTOR and KNOTTED1-LIKE HOMEOBOX families of transcription factors. Plant Physiology, 164 (4) : pp. 1952-1966.

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Quartile : Outlier, Sujet : PLANT SCIENCES

Abstract : KNOTTED1-LIKE HOMEOBOX (KNOX) genes are important regulators of meristem function, and a complex network of transcription factors ensures tight control of their expression. Here, we show that members of the GROWTH-REGULATING FACTOR (GRF) family act as players in this network. A yeast (Saccharomyces cerevisiae) one-hybrid screen with the upstream sequence of the KNOX gene Oskn2 from rice (Oryza sativa) resulted in isolation of OsGRF3 and OsGRF10. Specific binding to a region in the untranslated leader sequence of Oskn2 was confirmed by yeast and in vitro binding assays. ProOskn2:β-glucuronidase reporter expression was down-regulated by OsGRF3 and OsGRF10 in vivo, suggesting that these proteins function as transcriptional repressors. Likewise, we found that the GRF protein BGRF1 from barley (Hordeum vulgare) could act as a repressor on an intron sequence in the KNOX gene Hooded/Barley Knotted3 (Bkn3) and that AtGRF4, AtGRF5, and AtGRF6 from Arabidopsis (Arabidopsis thaliana) could repress KNOTTED-LIKE FROM ARABIDOPSIS THALIANA2 (KNAT2) promoter activity. OsGRF overexpression phenotypes in rice were consistent with aberrant meristematic activity, showing reduced formation of tillers and internodes and extensive adventitious root/shoot formation on nodes. These effects were associated with down-regulation of endogenous Oskn2 expression by OsGRF3. Conversely, RNA interference silencing of OsGRF3, OsGRF4, and OsGRF5 resulted in dwarfism, delayed growth and inflorescence formation, and up-regulation of Oskn2. These data demonstrate conserved interactions between the GRF and KNOX families of transcription factors in both monocot and dicot plants.

Mots-clés Agrovoc : Oryza sativa, Hordeum vulgare, Expression des gènes, Méristème intercalaire, Transcription génique, Saccharomyces cerevisiae, Arabidopsis thaliana, Tallage

Classification Agris : F30 - Plant genetics and breeding
F62 - Plant physiology - Growth and development
U30 - Research methods

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Kuijt Suzanne J.H., Leiden University (NLD)
  • Greco Rafaella, Leiden University (NLD)
  • Agalou Adamantia, Leiden University (NLD)
  • Shao Jingxia, Leiden University (NLD)
  • 't Hoen Corine C.J., Leiden University (NLD)
  • Övernäs Elin, Leiden University (NLD)
  • Osnato Michela, University of Milan (ITA)
  • Curiale Serena, University of Milan (ITA)
  • Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
  • van Gulik Robert, Leiden University (NLD)
  • de Faria Maraschin Simone, Leiden University (NLD)
  • Atallah Mirna, Leiden University (NLD)
  • De Kam Rolf J., University of Leiden (NLD)
  • Lamers Gerda E.M., Leiden University (NLD)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Rossini Laura, University of Milan (ITA)
  • Meijer Annemarie H., University of Leiden (NLD)
  • Ouwerkerk Pieter B.F., Universiteit Leiden (NLD) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589263/)

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