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Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance

Kamiri Mourad, Stift Marc, Costantino Gilles, Dambier Dominique, Kabbage Tariq, Ollitrault Patrick, Froelicher Yann. 2018. Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance. Frontiers in Plant Science, 9:1557, 12 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection.

Mots-clés géographiques Agrovoc : Corse

Mots-clés libres : Citrus, Somatic hybrid, Tetraploid, SSR, SNP, Inheritance

Classification Agris : F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Kamiri Mourad
  • Stift Marc, University of Konstanz (DEU)
  • Costantino Gilles, INRA (FRA)
  • Dambier Dominique, CIRAD-BIOS-UMR AGAP (FRA)
  • Kabbage Tariq, Domaines Abbès Kabbage (MAR)
  • Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-9456-5517 - auteur correspondant
  • Froelicher Yann, CIRAD-BIOS-UMR AGAP (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589415/)

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