The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting

Lopez David, Ben Amira Maroua, Brown Daniel, Muries Beatriz, Brunel-Michac Nicole, Bourgerie Sylvain, Porcheron Benoit, Lemoine Remi, Chrestin Hervé, Mollison Ewan, Di Cola Alessandra, Frigerio Lorenzo, Julien Jean-Louis, Gousset-Dupont Aurélie, Fumanal Boris, Label Philippe, Pujade-Renaud Valérie, Auguin Daniel, Venisse Jean-Stéphane. 2016. The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting. Plant Molecular Biology, 91 (4-5) : pp. 375-396.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation.

Mots-clés Agrovoc : Hevea brasiliensis, Latex, Relation plante eau, Génome, Homéostasie, Glycérol, Phylogénie, Saignée

Mots-clés géographiques Agrovoc : Côte d'Ivoire

Mots-clés libres : XIP aquaporin, Hevea brasiliensis, Latex, Evolution, Glycerol, Cell homeostasis

Classification Agris : F30 - Plant genetics and breeding
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 2 (2014-2018) - Valorisation de la biomasse

Auteurs et affiliations

  • Lopez David, Université Blaise Pascal (FRA)
  • Ben Amira Maroua, INRA (FRA)
  • Brown Daniel, University of Warwick (GBR)
  • Muries Beatriz, UCL (BEL)
  • Brunel-Michac Nicole, Université Blaise Pascal (FRA)
  • Bourgerie Sylvain, Université d'Orléans (FRA)
  • Porcheron Benoit, CNRS (FRA)
  • Lemoine Remi, CNRS (FRA)
  • Chrestin Hervé, IRD (FRA)
  • Mollison Ewan, TARRC (GBR)
  • Di Cola Alessandra, TARRC (GBR)
  • Frigerio Lorenzo, University of Warwick (GBR)
  • Julien Jean-Louis, Université Blaise Pascal (FRA)
  • Gousset-Dupont Aurélie, Université de Clermont-Ferrand (FRA)
  • Fumanal Boris, Université Blaise Pascal (FRA)
  • Label Philippe, Université Blaise Pascal (FRA)
  • Pujade-Renaud Valérie, CIRAD-BIOS-UMR AGAP (FRA)
  • Auguin Daniel, Université d'Orléans (FRA) - auteur correspondant
  • Venisse Jean-Stéphane, INRA (FRA) - auteur correspondant

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