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Salt-inducible expression of OsJAZ8 improves resilience against salt-stress

Peethambaran Preshobha K., Glenz René, Höninger Sabrina, Islam S. M. Shahinul, Hummel Sabine, Harter Klaus, Kolukisaoglu Üner, Meynard Donaldo, Guiderdoni Emmanuel, Nick Peter, Riemann Michael. 2018. Salt-inducible expression of OsJAZ8 improves resilience against salt-stress. BMC Plant Biology, 18:311, 15 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : Background: Productivity of important crop rice is greatly affected by salinity. The plant hormone jasmonate plays a vital role in salt stress adaptation, but also evokes detrimental side effects if not timely shut down again. As novel strategy to avoid such side effects, OsJAZ8, a negative regulator of jasmonate signalling, is expressed under control of the salt-inducible promoter of the transcription factor ZOS3–11, to obtain a transient jasmonate signature in response to salt stress. To modulate the time course of jasmonate signalling, either a full-length or a dominant negative C-terminally truncated version of OsJAZ8 driven by the ZOS3–11 promoter were expressed in a stable manner either in tobacco BY-2 cells, or in japonica rice. Results: The transgenic tobacco cells showed reduced mortality and efficient cycling under salt stress adaptation. This was accompanied by reduced sensitivity to Methyl jasmonate and increased responsiveness to auxin. In the case of transgenic rice, the steady-state levels of OsJAZ8 transcripts were more efficiently induced under salt stress compared to the wild type, this induction was more pronounced in the dominant-negative OsJAZ8 variant. Conclusions: The result concluded that, more efficient activation of OsJAZ8 was accompanied by improved salt tolerance of the transgenic seedlings and demonstrates the impact of temporal signatures of jasmonate signalling for stress tolerance.

Mots-clés Agrovoc : Oryza sativa, Tolérance au sel, Salinité du sol, Adaptation physiologique

Mots-clés libres : Rice, MeJa, JAZ, Salinity challenge, Jasmonate

Classification Agris : F60 - Plant physiology and biochemistry
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Peethambaran Preshobha K., Karlsruhe Institute of Technology (DEU)
  • Glenz René, Karlsruhe Institute of Technology (DEU)
  • Höninger Sabrina, Karlsruhe Institute of Technology (DEU)
  • Islam S. M. Shahinul, Karlsruhe Institute of Technology (DEU)
  • Hummel Sabine, University of Tübingen (DEU)
  • Harter Klaus, University of Tübingen (DEU)
  • Kolukisaoglu Üner, University of Tübingen (DEU)
  • Meynard Donaldo, CIRAD-BIOS-UMR AGAP (FRA)
  • Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
  • Nick Peter, Karlsruhe Institute of Technology (DEU)
  • Riemann Michael, Karlsruhe Institute of Technology (DEU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591293/)

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