Expression of DREB-like genes in Coffea canephora and C. arabica subjected to various types of abiotic stress

Ferreira Torres Luana, Reichel Tharyn, Dechamp Eveline, De Aquino Oliveira Sinara, Duarte Karoline Estefani, Costa Alves Gabriel Sergio, Tadeu Silva Anderson, Guitton Cotta Michelle, Santos Costa Tatiana, Cardamone Diniz Leandro Eugenio, Breitler Jean-Christophe, Collin Myriam, Vilela Paiva Luciano, Carvalho Andrade Alan, Etienne Hervé, Marraccini Pierre. 2019. Expression of DREB-like genes in Coffea canephora and C. arabica subjected to various types of abiotic stress. Tropical Plant Biology, 12 (2) : pp. 98-116.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : PLANT SCIENCES

Abstract : The aim of this work was to study the regulation of coffee DREB-like genes in leaves of C. arabica subjected to cold, heat, low relative humidity, exogenous abscisic acid and high light stress, as well as in leaves and roots of drought-tolerant and drought-susceptible clones of Coffea canephora subjected to water limitation. In C. arabica, CaERF017 was the most expressed gene under low temperatures and relative humidity, while low humidity and high temperatures up-regulated the expression of CaERF053 and CaERF014, respectively. Under water limitation, CcDREB1B, CcRAP2.4, CcERF027, CcDREB1D and CcTINY were the most expressed genes mainly in leaves of drought-tolerant C. canephora. On the other hand, expression of the CcERF016, CcRAP2.4 and CcDREB2F genes was highly up-regulated under water limitation in the roots of drought-susceptible C. canephora clone 22. We previously reported fine-tuned regulation of CcDREB1D promoter haplotypes (HP15, HP16 and HP17) in transgenic C. arabica subjected to low humidity. Here, we investigated the regulation of these haplotypes under high light, cold, heat, and abscisic acid (ABA) stress. In apical buds and leaf guard cells, GUS-stained percentages were higher in pHP16L-transformed plants subjected to low humidity, high light and ABA stress than in pHP17L- and pHP15L-transformed plants. We also reported up-regulated expression of the endogenous CaDREB1D gene for both the cold and low humidity in leaves of pHP16L-transformed C. arabica suggesting a key role of this gene in controlling the responses of coffee plants to abiotic stress probably through an ABA-dependent pathway.

Mots-clés Agrovoc : Coffea arabica, Coffea canephora, Expression des gènes, Résistance à la sécheresse

Mots-clés libres : Coffea canephora, Coffea arabica, Gene expression, Abiotic stresses, Promoter haplotypes

Classification Agris : F30 - Plant genetics and breeding
F60 - Plant physiology and biochemistry

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

  • Ferreira Torres Luana, UFLA (BRA)
  • Reichel Tharyn, EMBRAPA (BRA)
  • Dechamp Eveline, CIRAD-BIOS-UMR RPB (FRA)
  • De Aquino Oliveira Sinara, UFLA (BRA)
  • Duarte Karoline Estefani, UFLA (BRA)
  • Costa Alves Gabriel Sergio, UFLA (BRA)
  • Tadeu Silva Anderson, EMBRAPA (BRA)
  • Guitton Cotta Michelle, UFLA (BRA)
  • Santos Costa Tatiana, UFLA (BRA)
  • Cardamone Diniz Leandro Eugenio, EMBRAPA (BRA)
  • Breitler Jean-Christophe, CIRAD-BIOS-UMR IPME (MEX)
  • Collin Myriam, IRD (FRA)
  • Vilela Paiva Luciano, UFLA (BRA)
  • Carvalho Andrade Alan, UESC (BRA)
  • Etienne Hervé, CIRAD-BIOS-UMR IPME (FRA)
  • Marraccini Pierre, CIRAD-BIOS-UMR IPME (VNM) ORCID: 0000-0001-7637-6811 - auteur correspondant

Source : Cirad-Agritrop (

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