Development of a cost‐effective single nucleotide polymorphism genotyping array for management of greater yam germplasm collections

Cormier Fabien, Mournet Pierre, Causse Sandrine, Arnau Gemma, Maledon Erick, Gomez Rose-Marie, Pavis Claudie, Chaïr Hâna. 2019. Development of a cost‐effective single nucleotide polymorphism genotyping array for management of greater yam germplasm collections. Ecology and Evolution, 9 (10) : 5617-5636.

Quartile : Q2, Sujet : ECOLOGY / Quartile : Q3, Sujet : EVOLUTIONARY BIOLOGY

Résumé : Using genome‐wide single nucleotide polymorphism (SNP) discovery in greater yam (Discorea alata L.), 4,593 good quality SNPs were identified in 40 accessions. One hundred ninety six of these SNPs were selected to represent the overall dataset and used to design a competitive allele specific PCR array (KASPar). This array was validated on 141 accessions from the Tropical Plants Biological Resources Centre (CRB‐PT) and CIRAD collections that encompass worldwide D. alata diversity. Overall, 129 SNPs were successfully converted as cost‐effective genotyping tools. The results showed that the ploidy levels of accessions could be accurately estimated using this array. The rate of redundant accessions within the collections was high in agreement with the low genetic diversity of D. alata and its diversification by somatic clone selection. The overall diversity resulting from these 129 polymorphic SNPs was consistent with the findings of previously published studies. This KASPar array will be useful in collection management, ploidy level inference, while complementing accurate agro‐morphological descriptions.

Mots-clés Agrovoc : Dioscorea alata, Dioscoreaceae, génotype, marqueur génétique, collection de matériel génétique, polymorphisme génétique, nombre chromosomique, igname

Mots-clés géographiques Agrovoc : Guadeloupe, France

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Agences de financement européennes : European Commission

Auteurs et affiliations

• Cormier Fabien, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0002-7283-4616 - auteur correspondant
• Mournet Pierre, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8011-8647
• Causse Sandrine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-3877-8788
• Arnau Gemma, CIRAD-BIOS-UMR AGAP (GLP) ORCID: 0000-0003-0714-6830
• Maledon Erick, CIRAD-BIOS-UMR AGAP (GLP)
• Gomez Rose-Marie, INRA (GLP)
• Pavis Claudie, INRA (GLP)
• Chaïr Hâna, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/592306/)

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