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Insight into the microbial world of Bemisia tabaci cryptic species complex and its relationships with its host

Wang Hua-Ling, Lei Teng, Cameron Stephen L., Liu Yin-Quan, Zhang Zheng, Gowda Maruthi, De Barro Paul, Navas-Castillo Jesús, Omongo Christopher A., Delatte Hélène, Lee Kyeong-Yeoll, Patel Mitulkumar, Krause-Sakate Renate, Ng James, Wu San-Ling, Fiallo-Olivé Elvira, Liu Shu-Sheng, Colvin John, Wang Xiaowei. 2019. Insight into the microbial world of Bemisia tabaci cryptic species complex and its relationships with its host. Scientific Reports, 9:6568, 15 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Additional Information : Corrigendum paru dans Scientific Reports, (2019)1 p. https://doi.org/10.1038/s41598-019-42793-8

Abstract : The 37 currently recognized Bemisia tabaci cryptic species are economically important species and contain both primary and secondary endosymbionts, but their diversity has never been mapped systematically across the group. To achieve this, PacBio sequencing of full-length bacterial 16S rRNA gene amplicons was carried out on 21 globally collected species in the B. tabaci complex, and two samples from B. afer were used here as outgroups. The microbial diversity was first explored across the major lineages of the whole group and 15 new putative bacterial sequences were observed. Extensive comparison of our results with previous endosymbiont diversity surveys which used PCR or multiplex 454 pyrosequencing platforms showed that the bacterial diversity was underestimated. To validate these new putative bacteria, one of them (Halomonas) was first confirmed to be present in MED B. tabaci using Hiseq2500 and FISH technologies. These results confirmed PacBio is a reliable and informative venue to reveal the bacterial diversity of insects. In addition, many new secondary endosymbiotic strains of Rickettsia and Arsenophonus were found, increasing the known diversity in these groups. For the previously described primary endosymbionts, one Portiera Operational Taxonomic Units (OTU) was shared by all B. tabaci species. The congruence of the B. tabaci-host and Portiera phylogenetic trees provides strong support for the hypothesis that primary endosymbionts co-speciated with their hosts. Likewise, a comparison of bacterial alpha diversities, Principal Coordinate Analysis, indistinct endosymbiotic communities harbored by different species and the co-divergence analyses suggest a lack of association between overall microbial diversity with cryptic species, further indicate that the secondary endosymbiont-mediated speciation is unlikely to have occurred in the B. tabaci species group.

Mots-clés Agrovoc : Bemisia tabaci, Ravageur des plantes, Bacteria, Symbiote, PCR, Rickettsia, Différence biologique

Mots-clés complémentaires : microbiome, Halomonas, Arsenophonus

Classification Agris : H10 - Pests of plants
L60 - Animal taxonomy and geography

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Auteurs et affiliations

  • Wang Hua-Ling, Zhejiang University (CHN)
  • Lei Teng, Zhejiang University (CHN)
  • Cameron Stephen L., Purdue University (USA)
  • Liu Yin-Quan, Zhejiang University (CHN)
  • Zhang Zheng, Zhejiang University (CHN)
  • Gowda Maruthi, University of Greenwich (GBR)
  • De Barro Paul, CSIRO (AUS)
  • Navas-Castillo Jesús, Universidad de Málaga (ESP)
  • Omongo Christopher A., NaCRRI (UGA)
  • Delatte Hélène, CIRAD-BIOS-UMR PVBMT (REU)
  • Lee Kyeong-Yeoll, Kyungpook National University (KOR)
  • Patel Mitulkumar, University of Greenwich (GBR)
  • Krause-Sakate Renate, Universidade de São Paulo (BRA)
  • Ng James, UC (USA)
  • Wu San-Ling, Zhejiang University (CHN)
  • Fiallo-Olivé Elvira, NaCRRI (UGA)
  • Liu Shu-Sheng, Zhejiang University (CHN)
  • Colvin John, University of Greenwich (GBR) - auteur correspondant
  • Wang Xiaowei, Zhejiang University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/592916/)

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