Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta)

Flores Carolina, Zarate Carlos, Triplett Lindsay R., Maillot-Lebon Véronique, Moufid Yassine, Kanté Moussa, Bragard Claude, Verdier Valérie, Gagnevin Lionel, Szurek Boris, Robène Isabelle. 2019. Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta). Physiological and Molecular Plant Pathology, 105:n.spéc. Crop Pathology in Africa : 34-46.

https://doi.org/10.1016/j.pmpp.2018.07.005

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
593261.pdf
Télécharger (3MB) | Demander une copie

Quartile : Q2, Sujet : PLANT SCIENCES

Résumé : Cassava Bacterial Blight and Cassava Bacterial Necrosis are two bacterial diseases affecting cassava, respectively caused by Xanthomonas phaseoli pv. manihotis (Xpm) and Xanthomonas cassavae (Xc). Since both pathogens may be present on leaves and cause similar symptoms, we developed a new molecular diagnostic tool to detect and distinguish between Xpm and Xc. Based on genome sequences from the target species as well as from non-target species, in silico analysis was performed to select candidate primers through a novel strategy targeting specificity. Experimental validation enabled to establish a duplex-PCR diagnostic tool that will be useful for future surveillance programs and better disease control.

Mots-clés Agrovoc : Manihot esculenta, Xanthomonas campestris phaseoli, PCR, maladie bactérienne, épidémiologie

Mots-clés géographiques Agrovoc : Afrique

Mots-clés complémentaires : Xanthomonas cassavae, Xanthomonas phaseoli

Mots-clés libres : Cassava, Diagnostic PCR, Xanthomonas phaseoli pv. manihotis, Xanthomonas cassavae

Classification Agris : H20 - Maladies des plantes
U30 - Méthodes de recherche

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Auteurs et affiliations