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Unravelling the metabolic and hormonal machinery during key steps of somatic embryogenesis: A case study in coffee

Awada Rayan, Campa Claudine, Gibault Estelle, Dechamp Eveline, Georget Frederic, Lepelley Maud, Abdallah Cécile, Erban Alexander, Martinez-Seidel Federico, Kopka Joachim, Legendre Laurent, Leran Sophie, Conejero Geneviève, Verdeil Jean-Luc, Crouzillat Dominique, Breton David, Bertrand Benoît, Etienne Hervé. 2019. Unravelling the metabolic and hormonal machinery during key steps of somatic embryogenesis: A case study in coffee. International Journal of Molecular Sciences, 20 (19):4665, 29 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Abstract : Somatic embryogenesis (SE) is one of the most promising processes for large-scale dissemination of elite varieties. However, for many plant species, optimizing SE protocols still relies on a trial-and-error approach. Using coffee as a model plant, we report here the first global analysis of metabolome and hormone dynamics aiming to unravel mechanisms regulating cell fate and totipotency. Sampling from leaf explant dedifferentiation until embryo development covered 15 key stages. An in-depth statistical analysis performed on 104 metabolites revealed that massive re-configuration of metabolic pathways induced SE. During initial dedifferentiation, a sharp decrease in phenolic compounds and caffeine levels was also observed while auxins, cytokinins and ethylene levels were at their highest. Totipotency reached its highest expression during the callus stages when a shut-off in hormonal and metabolic pathways related to sugar and energetic substance hydrolysis was evidenced. Abscisic acid, leucine, maltotriose, myo-inositol, proline, tricarboxylic acid cycle metabolites and zeatin appeared as key metabolic markers of the embryogenic capacity. Combining metabolomics with multiphoton microscopy led to the identification of chlorogenic acids as markers of embryo redifferentiation. The present analysis shows that metabolite fingerprints are signatures of cell fate and represent a starting point for optimizing SE protocols in a rational way.

Mots-clés Agrovoc : Coffea, Embryogénèse somatique, Marqueur génétique, Différenciation cellulaire, Expression des gènes, Microscopie immunofluorescente, Microscopie

Mots-clés libres : Somatic embryogenesis, Metabolomics, Totipotency, Cell fate, Cell imaging, Coffee

Classification Agris : F30 - Plant genetics and breeding
U30 - Research methods

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Awada Rayan, CIRAD-BIOS-UMR IPME (FRA) - auteur correspondant
  • Campa Claudine, IRD (FRA)
  • Gibault Estelle, Nestlé (FRA)
  • Dechamp Eveline, CIRAD-BIOS-UMR RPB (FRA)
  • Georget Frederic, CIRAD-BIOS-UMR IPME (CRI)
  • Lepelley Maud, Nestlé (FRA)
  • Abdallah Cécile, Université de Montpellier (FRA)
  • Erban Alexander, Max Planck Institute of Molecular Plant Physiology (DEU)
  • Martinez-Seidel Federico, Max Planck Institute of Molecular Plant Physiology (DEU)
  • Kopka Joachim, Max Planck Institute of Molecular Plant Physiology (DEU)
  • Legendre Laurent, Université de Lyon (FRA)
  • Leran Sophie, CIRAD-BIOS-UMR IPME (FRA)
  • Conejero Geneviève, INRA (FRA)
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR AGAP (FRA)
  • Crouzillat Dominique, Nestlé (FRA)
  • Breton David, Centre de recherches Nestlé (FRA)
  • Bertrand Benoît, CIRAD-BIOS-UMR IPME (FRA)
  • Etienne Hervé, CIRAD-BIOS-UMR IPME (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593502/)

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