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Multiscale NMR investigations of two anatomically contrasted genotypes of sorghum under watered conditions and during drought stress

Sidi-Boulenouar Rahima, Cardoso Maida, Coillot Christophe, Rousset Sébastien, Nativel Eric, Charbit Alain, Baptiste Christelle, Alibert Eric, Gatineau Frédéric, Verdeil Jean-Luc, Goze-Bac Christophe. 2019. Multiscale NMR investigations of two anatomically contrasted genotypes of sorghum under watered conditions and during drought stress. Magnetic Resonance in Chemistry, 57 (9):n.spéc. Applications of magnetic resonance in food science : pp. 749-756.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : SPECTROSCOPY / Quartile : Q3, Sujet : CHEMISTRY, MULTIDISCIPLINARY / Quartile : Q3, Sujet : CHEMISTRY, PHYSICAL

Abstract : Today, in the presence of global warming, understanding how plants respond to drought stress is essential to meet the challenge of developing new cultivars and new irrigation strategies, consistent with the maintenance of crop productivity. In this context, the study of the relation between plants and water is of central interest for modeling their responses to biotic and abiotic constraints. Paradoxically, there are very few direct and noninvasive methods to quantify and measure the level and the flow of water in plants. The present work aims to develop a noninvasive methodology for living plant based on nuclear magnetic resonance (NMR) at low magnetic field and imaging (MRI) to tackle the issue of water quantity in plants. For this purpose, a portable NMR device measuring the signal level at 8 mT was built. This instrument addresses specific challenges such as miniaturization, accessibility, and overheating in order to maintain the plant intact of time over long period. Time dependence of the water content in sorghum plants is reported under abiotic stress as well as the fraction of transpirable soil water and the photosynthesis activity through the leaves. At high magnetic field (9.4 T), T2 maps were acquired on the same sorghum plants at two time points. The combination of these approaches allows us to identify ecophysiological biomarkers of drought stress. One particular interesting result concerns the spatial distribution of water in two anatomically contrasted sorghum genotypes.

Mots-clés Agrovoc : Sorghum bicolor, Stress dû à la sécheresse, Résistance à la sécheresse, Génotype, Spectroscopie RMN

Mots-clés libres : Abiotic stresses in plant, Ecophysiological biomarkers, NMR relaxation and imaging, Portable NMR setup, Radio frequency clipping coil

Classification Agris : F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding
U30 - Research methods

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Sidi-Boulenouar Rahima, Université de Montpellier (FRA) - auteur correspondant
  • Cardoso Maida, Université de Montpellier (FRA)
  • Coillot Christophe, Université de Montpellier (FRA)
  • Rousset Sébastien, Université de Montpellier (FRA)
  • Nativel Eric, Université de Montpellier (FRA)
  • Charbit Alain, Université de Montpellier (FRA)
  • Baptiste Christelle, CIRAD-BIOS-UMR AGAP (FRA)
  • Alibert Eric, Université de Montpellier (FRA)
  • Gatineau Frédéric, CIRAD-BIOS-UMR AGAP (FRA)
  • Verdeil Jean-Luc, CIRAD-BIOS-UMR AGAP (FRA)
  • Goze-Bac Christophe, UM2 (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593529/)

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