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Recombinant and native TviCATL from Trypanosoma vivax: Enzymatic characterisation and evaluation as a diagnostic target for animal African trypanosomosis

Eyssen Lauren E.-A., Vather Perina, Jackson Laurelle, Ximba Phindile, Biteau Nicolas, Baltz Théo, Boulangé Alain, Büscher Philippe, Coetzer Theresa. 2018. Recombinant and native TviCATL from Trypanosoma vivax: Enzymatic characterisation and evaluation as a diagnostic target for animal African trypanosomosis. Molecular and Biochemical Parasitology, 223 : pp. 50-54.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : PARASITOLOGY / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : African animal trypanosomosis (nagana) is caused by tsetse-transmitted protozoan parasites. Their cysteine proteases are potential chemotherapeutic and diagnostic targets. The N-glycosylated catalytic domain of Trypanosoma vivax cathepsin L-like cysteine protease, rTviCATLcat, was recombinantly expressed and purified from culture supernatants while native TviCATL was purified from T. vivax Y486 parasite lysates. Typical of Clan CA, family C1 proteases, TviCATL activity is sensitive to E-64 and cystatin and substrate specificity is defined by the S2 pocket. Leucine was preferred in P2 and basic and non-bulky, hydrophobic residues accepted in P1 and P3 respectively. Reversible aldehyde inhibitors, antipain, chymostatin and leupeptin, with Arg in P1 and irreversible peptidyl chloromethylketone inhibitors with hydrophobic residues in P2 inhibited TviCATL activity. TviCATL digested host proteins: bovine haemoglobin, serum albumin, fibrinogen and denatured collagen (gelatine) over a wide pH range, including neutral to slightly acidic pH. The recombinant catalytic domain of TviCATL showed promise as a diagnostic target for detecting T. vivax infection in cattle in an indirect antibody detection ELISA.

Mots-clés Agrovoc : Trypanosoma vivax, Trypanosomose, Test ELISA, Immunodiagnostic, Cystéine, Protéase

Mots-clés géographiques Agrovoc : Afrique au sud du Sahara, Amérique centrale, Amérique du Sud

Mots-clés libres : Trypanosomes, Cystein protease, Diagnostic, Trypanosoma vivax, Recombinant

Classification Agris : L73 - Animal diseases
L72 - Pests of animals

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Commission

Auteurs et affiliations

  • Eyssen Lauren E.-A., University of KwaZulu-Natal (ZAF)
  • Vather Perina, University of KwaZulu-Natal (ZAF)
  • Jackson Laurelle, University of KwaZulu-Natal (ZAF)
  • Ximba Phindile, University of KwaZulu-Natal (ZAF)
  • Biteau Nicolas, CNRS (FRA)
  • Baltz Théo, CNRS (FRA)
  • Boulangé Alain, CIRAD-BIOS-UMR INTERTRYP (FRA)
  • Büscher Philippe, Institute of tropical medecine (BEL)
  • Coetzer Theresa, University of KwaZulu-Natal (ZAF) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593620/)

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