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Proteomic landscape has revealed small rubber particles are crucial rubber biosynthetic machines for ethylene-stimulation in natural rubber production

Wang Dan, Xie Quanliang, Sun Yong, Tong Zheng, Chang Lili, Yu Li, Zhang Xueyan, Yuan Boxuan, He Peng, Jin Xuelin, Dong Yiyang, Li Hongbin, Montoro Pascal, Wang Xuchu. 2019. Proteomic landscape has revealed small rubber particles are crucial rubber biosynthetic machines for ethylene-stimulation in natural rubber production. International Journal of Molecular Sciences, 20:5082, 18 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Abstract : Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine.

Mots-clés Agrovoc : Hevea brasiliensis, Caoutchouc, Latex, Synthèse protéique, Phosphorylation, Éthylène

Mots-clés complémentaires : Protéomique

Mots-clés libres : Hevea brasiliensis, Natural rubber biosynthesis, Protein phosphorylation, Quantitative, Small rubber particle

Classification Agris : J11 - Handling, transport, storage and protection of plant products
J15 - Handling, transport, storage and protection of non-food or non-feed agricultural products

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Auteurs et affiliations

  • Wang Dan, Hainan University (CHN)
  • Xie Quanliang, Hainan University (CHN)
  • Sun Yong, CATAS (CHN)
  • Tong Zheng, CATAS (CHN)
  • Chang Lili, CATAS (CHN)
  • Yu Li, Hainan University (CHN)
  • Zhang Xueyan, Hainan University (CHN)
  • Yuan Boxuan, Hainan University (CHN)
  • He Peng, CATAS (CHN)
  • Jin Xuelin, Chinese Academy of Sciences (CHN)
  • Dong Yiyang, Beijing University (CHN)
  • Li Hongbin, Shihezi University (CHN)
  • Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)
  • Wang Xuchu, Hainan University (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593661/)

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