Agritrop
Home

Proteases as secreted exoproteins in mycoplasmas from ruminant lungs and their impact on surface-exposed proteins

Ganter Sarah, Miotello Guylaine, Manso-Silvan Lucia, Armengaud Jean, Tardy Florence, Gaurivaud Patrice, Thiaucourt François. 2019. Proteases as secreted exoproteins in mycoplasmas from ruminant lungs and their impact on surface-exposed proteins. Applied and Environmental Microbiology, 85 (23):e01439-19, 21 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
AEM01439-19-definitif-191023.pdf

Télécharger (895kB) | Request a copy

Quartile : Q1, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q2, Sujet : MICROBIOLOGY

Abstract : Many mycoplasma species are isolated from the ruminant lungs as either saprophytes or true pathogens. These wall-less bacteria possess a minimal genome and reduced metabolic capabilities. Accordingly, they rely heavily on their hosts for the supply of essential metabolites and, notably, peptides. Seven of 13 ruminant lung-associated Mycoplasma (sub)species were shown to possess caseinolytic activity when grown in rich media and assessed with a quantitative fluorescence test. For some species, this activity was detected in spent medium, an indication that proteases were secreted outside the mycoplasma cells. To identify these proteases, we incubated concentrated washed cell pellets in a defined medium and analyzed the supernatants by tandem mass spectrometry. Secreted-protease activity was detected mostly in the species belonging to the Mycoplasma mycoides cluster (MMC) and, to a lesser extent, in Mycoplasma bovirhinis. Analyzing a Mycoplasma mycoides subsp. capri strain, chosen as a model, we identified 35 expressed proteases among 55 predicted coding genes, of which 5 were preferentially found in the supernatant. Serine protease S41, acquired by horizontal gene transfer, was responsible for the caseinolytic activity, as demonstrated by zymography and mutant analysis. In an M. capricolum mutant, inactivation of the S41 protease resulted in marked modification of the expression or secretion of 17 predicted surface-exposed proteins. This is an indication that the S41 protease could have a role in posttranslational cleavage of surface-exposed proteins and ectodomain shedding, whose physiological impacts still need to be explored. IMPORTANCE Few studies pertaining to proteases in ruminant mycoplasmas have been reported. Here, we focus on proteases that are secreted outside the mycoplasma cell using a mass spectrometry approach. The most striking result is the identification, within the Mycoplasma mycoides cluster, of a serine protease that is exclusively detected outside the mycoplasma cells and is responsible for casein digestion. This protease may also be involved in the posttranslational processing of surface proteins, as suggested by analysis of mutants showing a marked reduction in the secretion of extracellular proteins. By analogy, this finding may help increase understanding of the mechanisms underlying this ectodomain shedding in other mycoplasma species. The gene encoding this protease is likely to have been acquired via horizontal gene transfer from Gram-positive bacteria and sortase-associated surface proteases. Whether this protease and the associated ectodomain shedding are related to virulence has yet to be ascertained.

Mots-clés Agrovoc : Mycoplasma mycoides, Ruminant, Maladie pulmonaire, Protéase, Protéine microbiologique, Virulence

Mots-clés complémentaires : Mycoplasma bovirhinis

Mots-clés libres : Mycoplasmas, Proteases, Secreted exoproteins, Virulence

Classification Agris : L73 - Animal diseases
L72 - Pests of animals

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Ganter Sarah, CIRAD-BIOS-UMR ASTRE (FRA)
  • Miotello Guylaine, CEA (FRA)
  • Manso-Silvan Lucia, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-5539-3804
  • Armengaud Jean, CEA (FRA)
  • Tardy Florence, ANSES (FRA)
  • Gaurivaud Patrice, ANSES (FRA)
  • Thiaucourt François, CIRAD-BIOS-UMR ASTRE (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/594726/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2020-10-10 ]