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A PCR, qPCR, and LAMP toolkit for the detection of the wheat blast pathogen in seeds

Thierry Maud, Chatet Axel, Fournier Elisabeth, Tharreau Didier, Ioos Renaud. 2020. A PCR, qPCR, and LAMP toolkit for the detection of the wheat blast pathogen in seeds. Plants, 9:227, 14 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Abstract : Wheat blast is a devastating disease caused by the pathogenic fungusPyricularia oryzae.Wheat blast first emerged in South America before more recently reaching Bangladesh. Even thoughthe pathogen can spread locally by air-dispersed spores, long-distance spread is likely to occur viainfected wheat seed or grain. Wheat blast epidemics are caused by a genetic lineage of the fungus,called theTriticumlineage, only differing from the otherP. oryzaelineages by less than 1% geneticdivergence. In order to prevent further spread of this pathogen to other wheat-growing areas inthe world, sensitive and specific detection tools are needed to test for contamination of traded seedlots by theP. oryzae Triticumlineage. In this study, we adopted a comparative genomics approach toidentify new loci specific to theP. oryzae Triticumlineage and used them to design a set of new markersthat can be used in conventional polymerase chain reaction (PCR), real-time PCR, or loop-mediatedisothermal amplification (LAMP) for the detection of the pathogen, with improved inclusivity andspecificity compared to currently available tests. A preliminary biological enrichment step of theseeds was shown to improve the sensitivity of the tests, which enabled the detection of the targetat an infection rate as low as 0.25%. Combined with others, this new toolkit may be particularlybeneficial in preventing the trade of contaminated seeds and in limiting the spread of the disease.

Mots-clés Agrovoc : blé, Champignon pathogène, Graine, génomique, genomic features [EN], Transmission des maladies, Spore, Distribution géographique

Mots-clés géographiques Agrovoc : Amérique latine, Brésil, Paraguay, Bolivie (État plurinational de), Argentine, Bangladesh

Mots-clés libres : Wheat, Wheat blast, Pyricularia oryzae, Diagnostic, Seed

Classification Agris : H20 - Plant diseases
F03 - Seed production and processing

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Thierry Maud, CIRAD-BIOS-UMR BGPI (FRA)
  • Chatet Axel, ANSES (FRA)
  • Fournier Elisabeth, INRAE (FRA)
  • Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
  • Ioos Renaud, ANSES (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595177/)

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