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Moniliophthora perniciosa development: Key genes involved in stress-mediated cell wall organization and autophagy

De Andrade Silva Edson Mario, Pereira Menezes Reis Sara, Argolo Caio Suzart, Santos Gomes Dayane, Santos Barbosa Ceslaine, Peres Gramacho Karina, Figueredo Ribeiro Lidiane, Santana Silva Raner José, Micheli Fabienne. 2020. Moniliophthora perniciosa development: Key genes involved in stress-mediated cell wall organization and autophagy. International Journal of Biological Macromolecules, 154 : pp. 1022-1035.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Abstract : Moniliophthora perniciosa is a basidiomycete responsible for the witches' broom disease in cacao (Theobroma cacao L.). Chitin synthase (CHS), chitinase (CHIT) and autophagy (ATG) genes have been associated to stress response preceding the formation of basidiocarp. An analysis of literature mining, interactomics and gene expression was developed to identify the main proteins related to development, cell wall organization and autophagy in M. perniciosa. TORC2 complex elements were identified and were involved in the response to the nutrient starvation during the fungus development stages preceding the basidiocarp formation. This complex interacted with target proteins related to cell wall synthesis and to polarization and cell division (FKS1, CHS, CDC42, ROM2). Autolysis and autophagy processes were associated to CHIT2, ATG8 and to the TORC1 complex (TOR1 and KOG1), which is central in the upstream signalization of the stress response due to nutrient starvation and growth regulation. Other important elements that participate to steps preceding basidiocarp formation were also identified (KOG1, SSZ1, GDI1, FKS1, CCD10, CKS1, CDC42, RHO1, AVO1, BAG7). Similar gene expression patterns during fungus reproductive structure formation and when treated by rapamycin (a nutritional related-autophagy stress agent) were observed: cell division related-genes were repressed while those related to autolysis/autophagy were overexpressed.

Mots-clés Agrovoc : Moniliophthora, Theobroma cacao, Expression des gènes, Carence en substance nutritive, Paroi cellulaire, Autolyse, Dégradation

Mots-clés géographiques Agrovoc : Amérique centrale, Amérique du Sud

Mots-clés complémentaires : Moniliophthora perniciosa

Mots-clés libres : Interactomics, Gene expression, Nutrient starvation

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • De Andrade Silva Edson Mario, UESC (BRA)
  • Pereira Menezes Reis Sara, UESC (BRA)
  • Argolo Caio Suzart, UESC (BRA)
  • Santos Gomes Dayane, UESC (BRA)
  • Santos Barbosa Ceslaine, UESC (BRA)
  • Peres Gramacho Karina, CEPLAC (BRA)
  • Figueredo Ribeiro Lidiane, UESC (BRA)
  • Santana Silva Raner José, UESC (BRA)
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595457/)

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