A standardised method of marking male mosquitoes with fluorescent dust

Culbert Nicole Jean, Kaiser Maria, Venter Nelius, Vreysen Marc J.B., Gilles Jérémie, Bouyer Jérémy. 2020. A standardised method of marking male mosquitoes with fluorescent dust. Parasites and Vectors, 13 (1):192, 11 p.

Url - jeu de données - Entrepôt autre : https://figshare.com/articles/journal_contribution/Additional_file_1_of_A_standardised_method_of_marking_male_mosquitoes_with_fluorescent_dust/12135249

Quartile : Q1, Sujet : PARASITOLOGY / Quartile : Q1, Sujet : TROPICAL MEDICINE

Résumé : Background: Prior to a major release campaign of sterile insects, including the sterile insect technique, male mosquitoes must be marked and released (small scale) to determine key parameters including wild population abundance, dispersal and survival. Marking insects has been routinely carried out for over 100 years; however, there is no gold standard regarding the marking of specific disease-transmitting mosquitoes including Anopheles arabiensis, Aedes aegypti and Aedes albopictus. The research presented offers a novel dusting technique and optimal dust colour and quantities, suitable for small-scale releases, such as mark-release-recapture studies. Methods: We sought to establish a suitable dust colour and quantity for batches of 100 male An. arabiensis, that was visible both by eye and under UV light, long-lasting and did not negatively impact longevity. A set of lower dust weights were selected to conduct longevity experiments with both Ae. aegypti and Ae. albopictus to underpin the optimal dust weight. A further study assessed the potential of marked male An. arabiensis to transfer their mark to undusted males and females. Results: The longevity of male An. arabiensis marked with various dust colours was not significantly reduced when compared to unmarked controls. Furthermore, the chosen dust quantity (5 mg) did not negatively impact longevity (P = 0.717) and provided a long-lasting mark. Dust transfer was found to occur from marked An. arabiensis males to unmarked males and females when left in close proximity. However, this was only noticeable when examining individuals under a stereomicroscope and thus deemed negligible. Overall, male Ae. aegypti and Ae. albopictus displayed a greater sensitivity to dusting. Only the lowest dust weight (0.5 mg) did not significantly reduce longevity (P = 0.888) in Ae. aegypti, whilst the lowest two dust weights (0.5 and 0.75 mg) had no significant impact on longevity (P = 0.951 and 0.166, respectively) in Ae. albopictus. Conclusion: We have devised a fast, inexpensive and simple marking method and provided recommended dust quantities for several major species of disease-causing mosquitoes. The novel technique provides an evenly distributed, long-lasting mark which is non-detrimental. Our results will be useful for future MRR studies, prior to a major release campaign.

Mots-clés Agrovoc : marquage, marqueur coloré, poudrage, lâcher d'insectes stériles, Anopheles arabiensis, Aedes albopictus, Aedes aegypti, vecteur de maladie, lutte anti-insecte, contrôle de maladies, animal mâle

Mots-clés libres : Technique de l'insecte stérile, Sterile Insect Technique, Lutte anti-vectorielle, Vector control

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Projets sur financement : (EU) Revolutionizing Insect Control

Auteurs et affiliations

• Culbert Nicole Jean, FAO (AUT) - auteur correspondant
• Kaiser Maria, National Institute for Communicable Diseases of the National Health Laboratory Service (ZAF)
• Venter Nelius, National Institute for Communicable Diseases of the National Health Laboratory Service (ZAF)
• Vreysen Marc J.B., FAO (AUT)
• Gilles Jérémie, FAO (AUT)
• Bouyer Jérémy, CIRAD-BIOS-UMR ASTRE (AUT) ORCID: 0000-0002-1913-416X

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595538/)

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