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Antifungal activity of Brazilian red propolis extract and isolation of bioactive fractions by thin-layer chromatography-bioautography

Dudoit Auriane, Mertz Christian, Chillet Marc, Cardinault Nicolas, Brat Pierre. 2020. Antifungal activity of Brazilian red propolis extract and isolation of bioactive fractions by thin-layer chromatography-bioautography. Food Chemistry, 327:127060, 9 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Abstract : Objectives: This study set out to highlight the in vitro and in vivo antifungal activity of an Ethanolic Extract of Red Brazilian Propolis (EERBP) and identify bioactive fractions effective against Colletotrichum musae. Methods: Active fractions were detected by the thin-layer chromatography-bioautography method and characterised by HPLC-MSn. Results: The in vitro results showed that EERBP had strong antifungal properties against C. musae (81 ± 1% inhibition at 1.6 g GAE L−1). Medicarpin, (3S)-vestitol and (3S)-neovestitol were the main compounds identified in the EERBP extract (45% of all detected peaks). Two isolated fractions displayed inhibition percentages of 35 ± 4 and 42 ± 1%, respectively, on C. musae mycelial growth compared to the EERBP extract. The biological activity of the two fractions displayed an additive effect. Conclusion: A further in vivo investigation revealed that EERBP is a potential natural alternative for controlling banana crown rot.

Mots-clés Agrovoc : Propriété antifongique, Propolis, Chromatographie en couche mince

Mots-clés complémentaires : Colletotrichum musae

Mots-clés libres : Propolis, Antifungal, Polyphenols, Thin-layer chromatography-bioautography, Colletotrichum musae, Banana

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Dudoit Auriane, Université de Montpellier (FRA)
  • Mertz Christian, CIRAD-PERSYST-UMR Qualisud (FRA)
  • Chillet Marc, CIRAD-PERSYST-UMR Qualisud (REU)
  • Cardinault Nicolas, Société Pollenergie (FRA)
  • Brat Pierre, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-0429-9575 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595819/)

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