Boosting the sterile insect technique with pyriproxyfen increases tsetse flies Glossina palpalis gambiensis sterilization in controlled conditions

Laroche Lison, Ravel Sophie, Baldet Thierry, Lancelot Renaud, Chandre Fabrice, Rossignol Marie, Le Goff Voahirana, Duhayon Maxime, Fafet Jean-François, Parker Andrew Gordon, Bouyer Jérémy. 2020. Boosting the sterile insect technique with pyriproxyfen increases tsetse flies Glossina palpalis gambiensis sterilization in controlled conditions. Scientific Reports, 10:9947, 11 p.

https://doi.org/10.1038/s41598-020-66850-9

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Type d'url non précisé : https://rdcu.be/b5aO6

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Tsetse flies (Diptera: Glossinidae) are the main vectors of animal and human trypanosomoses in Africa. The Sterile Insect Technique (SIT) has proven effective in controlling tsetse flies when applied to isolated populations but necessitates the production of large numbers of sterile males. A new approach, called boosted SIT, combining SIT with the contamination of wild females by sterile males coated with biocides has been proposed for large-scale control of vector populations. The aim of the study was to evaluate this new approach using pyriproxyfen on the riverine species Glossina palpalis gambiensis (Vanderplank, 1949) in the laboratory. The contamination dose and persistence of pyriproxyfen on sterile males, the impact of pyriproxyfen on male survival, and the dynamics of pyriproxyfen transfer from a sterile male to a female during mating, as well as the impact of pyriproxyfen on pupal production and adult emergence, were evaluated in the laboratory. For this purpose, a method of treatment by impregnating sterile males with a powder containing 40% pyriproxyfen has been developed. The results showed that the pyriproxyfen has no impact on the survival of sterile males. Pyriproxyfen persisted on sterile males for up to 10 days at a dose of 100 ng per fly. In addition, the horizontal transfer of pyriproxyfen from a treated sterile male to a female during mating could be measured with an average of 50 ng of pyriproxyfen transferred. After contacts without mating, the average quantity transferred was more than 10 ng. Finally, the pyriproxyfen powder was very effective on G. p. gambiensis leading to 0% emergence of the pupae produced by contaminated females. These promising results must be confirmed in the field. A large-scale assessment of this boosted pyriproxyfen-based SIT approach will be carried out against tsetse flies in Senegal (West Africa).

Mots-clés Agrovoc : stérilisation, lutte biologique, lâcher d'insectes stériles

Mots-clés complémentaires : Glossina palpalis gambiensis, pyriproxifène

Classification Agris : L72 - Organismes nuisibles des animaux
L53 - Physiologie animale - Reproduction

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Projets sur financement : (EU) Revolutionizing Insect Control

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