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Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities

Duong Benoit, Nguyen Xuan Hoa, Phan Viet Ha, Colella Stefano, Trinh Quang Phap, Hoang Thi Giang, Nguyen Thi Tuyet, Marraccini Pierre, Lebrun Michel, Duponnois Robin. 2021. Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities. Microbiological Research, 242:126613, 13 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Abstract : The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea canephora and Coffea liberica, twelve were reported for the first time in endophytic association with coffee including members of the genera Brachybacterium, Caballeronia, Kitasatospora, Lechevalieria, Leifsonia, Luteibacter, Lysinibacillus, Mycolicibacterium, Nakamurella, Paracoccus, Sinomonas and Sphingobium. A total of eighty bacterial endophytes were characterized in vitro for several plant growth promoting and biocontrol traits including: the phosphate solubilization, the indolic compounds, siderophores, HCN, esterase, lipase, gelatinase and chitinase production. A subset of fifty selected bacteria were tested for their potential as biocontrol agents with in vitro confrontations with the fungal pathogen Fusarium oxysporum as well as the coffee parasitic nematodes Radopholus duriophilus and Pratylenchus coffeae. The three most efficient isolates on F. oxysporum belonging to the Bacillus, Burkholderia, and Streptomyces genera displayed a growth inhibition rate higher than 40%. Finally, five isolates from the Bacillus genus were able to lead to 100% of mortality in 24 h on both R. duriophilus and P. coffeae.

Mots-clés Agrovoc : Coffea canephora, Coffea liberica, Endophyte, Bacteria, Symbiote, Biopesticide, Nématocide, Propriété antifongique, Croissance

Mots-clés géographiques Agrovoc : Viet Nam

Mots-clés complémentaires : bactérie endophyte, propriété nématocide

Mots-clés libres : Coffee, Endosymbiosis, Antifungal activity, Biocontrol, Endophytes, Nematicidal activity, Plant growth-promoting

Classification Agris : H10 - Pests of plants
H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Duong Benoit, Université de Montpellier (FRA)
  • Nguyen Xuan Hoa, WASI (VNM)
  • Phan Viet Ha, WASI (VNM)
  • Colella Stefano, INRAE (FRA)
  • Trinh Quang Phap, VAST (VNM)
  • Hoang Thi Giang, Université de Montpellier (FRA)
  • Nguyen Thi Tuyet, VAAS (VNM)
  • Marraccini Pierre, CIRAD-BIOS-UMR IPME (VNM) ORCID: 0000-0001-7637-6811
  • Lebrun Michel, Université de Montpellier (FRA)
  • Duponnois Robin, IRD (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/596668/)

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