Selection of natural extracts for their antioxidant capacity by using a combination of in Vitro assays

Deyrieux Charlotte, Durand Erwann, Guillou Sonia, Barouh Nathalie, Baréa Bruno, Salaun Françoise Michel, Villeneuve Pierre. 2020. Selection of natural extracts for their antioxidant capacity by using a combination of in Vitro assays. Journal of the American Oil Chemists' Society, 97 (11) : pp. 1229-1241.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Abstract : Numerous different in vitro assays, labeled as “antioxidant assays,” are used intensively to predict the antioxidant capacity of phenolic compounds. Most of these methods give valuable information in terms of chemical reactivities but also present some weaknesses. It may be difficult to use them to predict antioxidant capacity in real conditions. Indeed, lipid oxidation is a complex reaction, with numerous paths and components, and antioxidant action can occur via a multitude of mechanisms, especially when different phases coexist in the lipid‐based formulation. Yet, correctly combining selected in vitro methods to extract complementary information with respect to antioxidant behaviors would be much better and will help reducing the gap between prediction and efficacy in the finished product. Thus, we hereby propose a methodology to evaluate the antioxidant properties of 12 selected natural polyphenolic extracts based on the appropriate combination of in vitro assays. The scores obtained with the DPPH test, the measure of the chelation capacity, the evaluation of antioxidants efficiency in emulsions (CAT and VESICAT assays), or in bulk oils were submitted to a statistical treatment. This analysis allowed a ranking on their global antioxidant capacities and the creation of clusters depending on their mechanisms of action and the type of media where the tests were performed.

Mots-clés Agrovoc : Antioxydant, Méthode d'essai, Expérimentation, Composé phénolique, Produit végétal, Expérimentation in vitro, Sélection in vitro, Polyphénol, Oxydation, Lipide

Mots-clés libres : Lipides, Antioxydants, Oxydation, Polyphenols, Lipid oxidation

Classification Agris : Q04 - Food composition
F60 - Plant physiology and biochemistry
U30 - Research methods

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations

  • Deyrieux Charlotte, Université de Montpellier (FRA)
  • Durand Erwann, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0002-0306-8081
  • Guillou Sonia, Diana Pet Food (FRA)
  • Barouh Nathalie, CIRAD-PERSYST-UMR IATE (FRA)
  • Salaun Françoise Michel, Diana Pet Food (FRA)
  • Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494 - auteur correspondant

Source : Cirad-Agritrop (

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