Genotyping-by-sequencing technology in plant taxonomy and phylogeny

Favre Félicien, Jourda Cyril, Besse Pascale, Charron Carine. 2021. Genotyping-by-sequencing technology in plant taxonomy and phylogeny. In : Molecular plant taxonomy: method and protocols. Besse Pascale (ed.). New York : Humana Press, 167-178. (Methods in Molecular Biology, 2222) ISBN 978-1-0716-0996-5

https://doi.org/10.1007/978-1-0716-0997-2_10

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Résumé : Genotyping-by-sequencing (GBS) is a method to discover and genotype simultaneous genome-wide high-throughput single nucleotide polymorphisms (SNPs). GBS is based on reducing genome complexity with restriction enzymes. Here we describe a method developed by Elshire et al. for constructing simplified GBS libraries and recent bioinformatic approaches developed to analyze the large volume of polymorphism data generated by this method. GBS approach is suitable for population studies, taxonomic and phylogenic studies, germplasm characterization, and breeding and trait mapping for a wide range of organisms, including plants with complex genomes.

Mots-clés Agrovoc : phylogénie, génotype, taxonomie, germoplasme, amélioration des plantes, génotypage, génotypage par séquençage

Mots-clés libres : Genotyping by sequencing, Next-generation sequencing, SNP, Plant diversity markers

Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie

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