Genetic diversity of native and cultivated Ugandan Robusta coffee (Coffea canephora Pierre ex A. Froehner): Climate influences, breeding potential and diversity conservation

Kiwuka Catherine, Goudsmit Eva, Tournebize Rémi, Oliveira de Aquino Sinara, Douma Jacob C., Bellanger Laurence, Crouzillat Dominique, Stoffelen Piet, Sumirat Ucu, Legnaté Hyacinthe, Marraccini Pierre, De Kochko Alexandre, Carvalho Andrade Alan, Wasswa Mulumba John, Musoli Pascal, Anten Niels P.R., Poncet Valérie. 2021. Genetic diversity of native and cultivated Ugandan Robusta coffee (Coffea canephora Pierre ex A. Froehner): Climate influences, breeding potential and diversity conservation. PloS One, 16 (2):e0245965, 19 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Kiwuka et al. PLoS ONE 2021.pdf

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Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie; Staps

Abstract : Wild genetic resources and their ability to adapt to environmental change are critically important in light of the projected climate change, while constituting the foundation of agricultural sustainability. To address the expected negative effects of climate change on Robusta coffee trees (Coffea canephora), collecting missions were conducted to explore its current native distribution in Uganda over a broad climatic range. Wild material from seven forests could thus be collected. We used 19 microsatellite (SSR) markers to assess genetic diversity and structure of this material as well as material from two ex-situ collections and a feral population. The Ugandan C. canephora diversity was then positioned relative to the species' global diversity structure. Twenty-two climatic variables were used to explore variations in climatic zones across the sampled forests. Overall, Uganda's native C. canephora diversity differs from other known genetic groups of this species. In northwestern (NW) Uganda, four distinct genetic clusters were distinguished being from Zoka, Budongo, Itwara and Kibale forests A large southern-central (SC) cluster included Malabigambo, Mabira, and Kalangala forest accessions, as well as feral and cultivated accessions, suggesting similarity in genetic origin and strong gene flow between wild and cultivated compartments. We also confirmed the introduction of Congolese varieties into the SC region where most Robusta coffee production takes place. Identified populations occurred in divergent environmental conditions and 12 environmental variables significantly explained 16.3% of the total allelic variation across populations. The substantial genetic variation within and between Ugandan populations with different climatic envelopes might contain adaptive diversity to cope with climate change. The accessions that we collected have substantially enriched the diversity hosted in the Ugandan collections and thus contribute to ex situ conservation of this vital genetic resource. However, there is an urgent need to develop strategies to enhance complementary in-situ conservation of Coffea canephora in native forests in northwestern Uganda.

Mots-clés Agrovoc : Coffea canephora, Organisme indigène, Variation génétique, Distribution des populations, Distribution géographique, Marqueur génétique, Microsatellite, Zone climatique

Mots-clés géographiques Agrovoc : Ouganda

Mots-clés libres : Coffea canephora, Genetic diversity, Uganda, Microsatellite

Classification Agris : F30 - Plant genetics and breeding

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Auteurs et affiliations

  • Kiwuka Catherine, NARO (UGA)
  • Goudsmit Eva, Wageningen University (NLD)
  • Tournebize Rémi, IRD (FRA)
  • Oliveira de Aquino Sinara, IRD (FRA)
  • Douma Jacob C., Wageningen University (NLD)
  • Bellanger Laurence, Nestlé (FRA)
  • Crouzillat Dominique, Nestlé (FRA)
  • Stoffelen Piet, Botanic Garden Meise (BEL)
  • Sumirat Ucu, ICCRI (IDN)
  • Legnaté Hyacinthe, CNRA (CIV)
  • Marraccini Pierre, CIRAD-BIOS-UMR IPME (VNM) ORCID: 0000-0001-7637-6811
  • De Kochko Alexandre, IRD (FRA)
  • Carvalho Andrade Alan, EMBRAPA (BRA)
  • Wasswa Mulumba John, NARO (UGA)
  • Musoli Pascal, NARO (UGA)
  • Anten Niels P.R., Wageningen University (NLD)
  • Poncet Valérie, IRD (FRA) - auteur correspondant

Source : Cirad-Agritrop (

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