Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao)

Muller Emmanuelle, Ullah Ihsa, Dunwell Jim M., Daymond Andrew J., Richardson Megan, Allainguillaume Joel, Wetten Andrew. 2021. Identification and distribution of novel badnaviral sequences integrated in the genome of cacao (Theobroma cacao). Scientific Reports, 11:8270, 13 p.

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Theobroma cacao is one of the most economically important tropical trees, being the source of chocolate. As part of an ongoing study to understand the diversity of the badnavirus complex, responsible for the cacao swollen shoot virus disease in West Africa, evidence was found recently of virus-like sequences in asymptomatic cacao plants. The present study exploited the wealth of genomic resources in this crop, and combined bioinformatic, molecular, and genetic approaches to report for the first time the presence of integrated badnaviral sequences in most of the cacao genetic groups. These sequences, which we propose to name eTcBV for endogenous T. cacao bacilliform virus, varied in type with each predominating in a specific genetic group. A diagnostic multiplex PCR method was developed to identify the homozygous or hemizygous condition of one specific insert, which was inherited as a single Mendelian trait. These data suggest that these integration events occurred before or during the species diversification in Central and South America, and prior to its cultivation in other regions. Such evidence of integrated sequences is relevant to the management of cacao quarantine facilities and may also aid novel methods to reduce the impact of such viruses in this crop.

Mots-clés Agrovoc : indexage des virus des plantes, Theobroma cacao, héritabilité génotypique, séquence nucléotidique

Mots-clés complémentaires : Badnavirus, viral genomes (en)

Mots-clés libres : Theobroma cacao, Badnavirus, Viral integration

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

• Muller Emmanuelle, CIRAD-BIOS-UMR AGAP (FRA)
• Ullah Ihsa, University of Reading (GBR)
• Dunwell Jim M., University of Reading (GBR) - auteur correspondant
• Daymond Andrew J., University of Reading (GBR)
• Richardson Megan, University of the West of England (GBR)
• Allainguillaume Joel, University of the West of England (GBR)
• Wetten Andrew, University of the West of England (GBR)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598092/)

Métriques

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