Boonrumpun Prapon, Pannetier Catherine, Chupeau Yves. 1997. Isolation and culture of protoplasts from different explants of cotton (Gossypium hirsutum L.). Kasetsart Journal. Natural Sciences, 31 (5) : 55-59.
Autre titre : Isolement et culture de protoplastes de différents explants de coton (Gossypium hirsutum L.)
Résumé : Protoplasts were isolated from callus, cotyledons, hypocotyls and leaves of cotton (Gossypium hirsutum L. var. Coker 310) using different digestion media. Protoplast yield per gram of fresh mass of leaves, callus cotyledons and hypocotyls was 22.2, 5.5, 1.7 and 1. 1 million respectively. Protoplasts were most viable in basic medium in presence of thidiazuro 0.663 gm/1 and 2,4,5-trichlorophenoxy acetic acid 7 mg/1. Hypocotyl and leaf-derived protoplasts formed microcolonies. Addition of CaC12 and glutamine to the basic medium improved protoplast survival, and division of hypocotyl and leaf-derived protoplasts. The growth conditions, such as the light and carbon source of donor plant were the important factors for protoplast isolation and culture.
Mots-clés Agrovoc : Gossypium hirsutum, explant, protoplaste, cal, cotylédon, hypocotyle, feuille, culture de cellule
Classification Agris : F02 - Multiplication végétative des plantes
Auteurs et affiliations
- Boonrumpun Prapon, DORAS (THA)
- Pannetier Catherine, CIRAD-CA-COTON (FRA)
- Chupeau Yves, INRA (FRA)
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : CD_BR10003 [(Bibliothèque de Lavalette)] ; CD_BR8562 [(Bibliothèque de Lavalette)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/6562/)
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