Agritrop
Accueil

Genetic diversity among Frankia strains nodulating members of the family Casuarinaceae in Australia revealed by PCR and restriction fragment length polymorphism analysis with crushed root nodules

Rouvier Carole, Prin Yves, Reddell Paul, Normand Philippe, Simonet Pascal. 1996. Genetic diversity among Frankia strains nodulating members of the family Casuarinaceae in Australia revealed by PCR and restriction fragment length polymorphism analysis with crushed root nodules. Applied and Environmental Microbiology, 62 (3) : 979-985.

Article de revue ; Article de revue à facteur d'impact
[img]
Prévisualisation
Version publiée - Anglais
Utilisation soumise à autorisation de l'auteur ou du Cirad.
389876.pdf

Télécharger (260kB) | Prévisualisation

Autre titre : Diversité génétique entre les organes de nodulation des souches de Frankia de la famille de Casuarinaceae en Australie révélée par la PCR et l'analyse du polymorphisme de la longueur des fragments de restriction à partir des nodules racinaires broyés

Résumé : DNA extracted directly from nodules was used to assess the genetic diversity of #Frankia# strains symbiotically associated with two species of genus #Casuarina# and two of the genus #Allocasuarina# naturally occuring in northeastern Australia. DNA from field-collected nodules or extracted from reference cultures of #Casuarina#-infective #Frankia# strains was used as the template in PCRs with primers targeting two DNA regions, one in the ribosomal operon and the other in the #nif# operon. PCR products were then analysed by using a set of restriction andonucleases. Five distinct genetic groups were recognized on the basis of these restriction patterns. These groups were consistently associated with the host species from which the nodules originated. All isolated reference strains had similar patterns and were assigned to group 1 along with six of eight unisolated #Frankia# strains from #Casuarina equisetifolia# in Australia. Group 2 consisted of two unisolated #Frankia# strains from #C. equisetifolia#, whereas groups 3 to 5 comprised all unisolated strains from #Casuarina cunninghamiana#, #Allocasuarina torulosa#, and #Allocasuarina littoralis#, respectively. These results demonstrate that, contrary to the results previous molecular studies of isolated strains, there is genetic diversity among #Frankia# strains that infect members of the family Casuarinaceae. The apparent high homogeneity of #Frankia# strains in these previous studies probably relates to the single host species from which the strains were obtained and the origin of these strains from areas outside the natural geographic range of members of the family Casuarinaceae, where genetic diversity could be lower than in Australia.

Mots-clés Agrovoc : Frankia, Casuarinaceae, biodiversité, adn, variation génétique, nodosité racinaire, PCR, RFLP

Mots-clés géographiques Agrovoc : Australie

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

  • Rouvier Carole
  • Prin Yves, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA) ORCID: 0000-0002-3706-0045
  • Reddell Paul
  • Normand Philippe
  • Simonet Pascal

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389876/)

Voir la notice (accès réservé à Agritrop) Voir la notice (accès réservé à Agritrop)

[ Page générée et mise en cache le 2024-03-13 ]