Genetically modified coffee trees resistant to the coffee leaf miner, Perileucoptera coffeella

Leroy Thierry, Henry A.M., Philippe René, Royer Monique, Deshayes Alain, Frutos Roger, Duris Daniel, Dufour Magali, Tessereau Sylviane, Jourdan Isabelle, Bossard G., Lacombe Catherine, Fenouillet Catherine. 1999. Genetically modified coffee trees resistant to the coffee leaf miner, Perileucoptera coffeella. In : Biotechnology of #Bacillus thuringiensis# = [Biotechnologie de #Bacillus thuringiensis#]. Ziniu Y. (ed.), Ming S. (ed.), Ziduo L. (ed). Beijing : Science Press, p. 197. Pacific Rim Conference on Biotechnology. 3, Wuhan, Chine, 5 October 1999/9 October 1999.

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Autre titre : Transformation génétique de caféiers pour la résistance à la mineuse des feuilles Perileucoptera coffeella

Abstract : A synthetic version of the Bacillus thuringiensis cry1Ac gene was used in transformation of coffee species (Coffea canephora and Coffea arabica) to confer resistance to an important pest in South America and East Africa, the coffee leaf miner (Perileucoptera coffeella and other Leucoptera spp.). Somatic embryos were co-cultivated with the LBA4404 strain of Agrobacterium tumefaciens containing the synthetic cry1Ac gene, the csr1-1 gene conferring resistance to a herbicide used as a selective agent, and the uidA gene. More than 100 transformed plants from independent transformation events have been obtained for each coffee genotype. The expression of the cry1Ac gene was assessed by western blot analyses, and effective resistance of transgenic plants against leaf miner was verified by bioassays with insects. Correct integration of the genes was analysed. Agronomic conformity of the transgenic plants will be evaluated in field planting in French Guyana, and a suitable insect management program will be implemented. These plants could represent a good opportunity to observe gene expression in a perennial crop and the management of resistance against an endocarpic insect with a protein from B. thuringiensis. (Texte intégral)

Mots-clés Agrovoc : Coffea canephora, Coffea arabica, Transformation génétique, Bacillus thuringiensis, Agrobacterium tumefaciens, Transfert de gène, Résistance aux organismes nuisibles, Perileucoptera coffeella, Leucoptera, Expression des gènes

Classification Agris : F30 - Plant genetics and breeding
H10 - Pests of plants

Auteurs et affiliations

  • Leroy Thierry, CIRAD-CP-CAFE (FRA)
  • Henry A.M., Centre de recherches Nestlé (FRA)
  • Philippe René, CIRAD-CP-COCOTIER (FRA)
  • Deshayes Alain, Centre de recherches Nestlé (FRA)
  • Duris Daniel, CIRAD-CP-CAFE (FRA)
  • Dufour Magali, CIRAD-CP-CAFE (FRA)
  • Tessereau Sylviane, Centre de recherches Nestlé (FRA)
  • Jourdan Isabelle, CIRAD-CP-CAFE (FRA)
  • Bossard G., Centre de recherches Nestlé (FRA)
  • Lacombe Catherine, CIRAD-CP-CAFE (FRA)
  • Fenouillet Catherine, CIRAD-CP-CAFE (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (

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