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The development of simple sequence repeat (SSR) markers for Magnaporthe grisae and their integration into an established genetic linkage map

Kaye Claudia, Milazzo Joëlle, Rozenfeld Sophie, Lebrun Marc-Henri, Tharreau Didier. 2003. The development of simple sequence repeat (SSR) markers for Magnaporthe grisae and their integration into an established genetic linkage map. Fungal Genetics and Biology, 40 (3) : 207-214.

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Résumé : Although microsatellite or simple sequence repeat (SSR) markers have several advantages, few have been developed in fungi. The goal of this study was to identify and characterize SSR-containing loci in the filamentous ascomycete Magnaporthe grisea, the causal agent of rice blast disease, and to add these markers to an integrated genetic map of this species [Theor. Appl. Genet. 95 (1997) 20]. We have constructed and screened a microsatellite-enriched small-insert genomic library as well as exploited both publicly available and one proprietary databases for identification of M. grisea SSR containing sequences. Twenty-four out of 49 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of six isolates. The number of alleles at each locus ranged from two to six when assayed on 30% agarose gels. Twenty-three of the primer pairs amplified polymorphic products between Guy 11 and 2539, the parents of a cross from which a genetic map for M. grisea has been established. Genetic analysis showed that all the markers segregated in the expected 1:1 ratio and map positions were determined for all 23 loci.

Mots-clés Agrovoc : Magnaporthe grisea, carte génétique, marqueur génétique, séquence nucléotidique

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

  • Kaye Claudia, CIRAD-CA-CALIM (FRA)
  • Milazzo Joëlle, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
  • Rozenfeld Sophie, Bayer (FRA)
  • Lebrun Marc-Henri, CNRS (FRA)
  • Tharreau Didier, CIRAD-CA-CALIM (FRA) ORCID: 0000-0003-3961-6120

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/519130/)

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