Two expansin cDNAs from #Prunus armeniaca" expressed during fruit ripening are differently regulated by ethylene

Mbéguié-A-Mbéguié Didier, Gouble Barbara, Gomez Rose-Marie, Audergon Jean-Marc, Albagnac Guy, Fils-Lycaon Bernard. 2002. Two expansin cDNAs from #Prunus armeniaca" expressed during fruit ripening are differently regulated by ethylene. Plant Physiology and Biochemistry, 40 (5) : 445-452.

Résumé : Little is known about gene expression during fruit ripening of apricot (Prunus armeniaca L. cv. Bergeron), especially for enzymes involved in cell wall modifications. A partial cDNA clone encoding a protein homologous to expansin was isolated from a ripe apricot fruit cDNA library. This clone was used to isolate two full-length expansin cDNAs, Pa-Exp1 (accession no. U93167) and Pa-Exp2 (accession no. AF038815) from the same cDNA library. The predicted polypeptides encoded by these two cDNAs are different and belong to the [alpha]-expansin family; Pa-Exp1 and Pa-Exp2 are two different members of a multigene family. These two clones are mostly expressed in fruit, during its ripening. Pa-Exp1 mRNA accumulated abundantly at the half-ripe stage of fruit development and decreased thereafter. Pa-Exp2 mRNA level increased from the immature-green stage to the half-ripe stage where it peaked before declining. During the ripening process, Pa-Exp1 and Pa-Exp2 gene expression appeared to be positively correlated with fruit size. Post-harvest treatments by air, ethylene, and 1-methyl cyclopropene led us to conclude that Pa-Exp1 appears to be developmentally down-regulated by ethylene while Pa-Exp2 is not affected. The relationship between Pa-Exp1, Pa-Exp2 and the softening process is also discussed.

Mots-clés Agrovoc : Prunus armeniaca, adn, paroi cellulaire, éthylène, expression des gènes, mûrissage, physiologie après récolte, enzyme, banque de gènes, stade de développement végétal, lutte après récolte

Classification Agris : F62 - Physiologie végétale - Croissance et développement
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Mbéguié-A-Mbéguié Didier, CIRAD-FLHOR-BPA (GLP) ORCID: 0000-0002-9256-3999
• Gouble Barbara, INRA (FRA)
• Gomez Rose-Marie, INRA (GLP)
• Audergon Jean-Marc, INRA (FRA)
• Albagnac Guy, INRA (FRA)
• Fils-Lycaon Bernard, INRA (GLP)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/520295/)

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