Using SD-AFLP and MSAP to assess CCGG methylation in the banana genome

Baurens Franc-Christophe, Bonnot François, Bienvenu David, Causse Sandrine, Legavre Thierry. 2003. Using SD-AFLP and MSAP to assess CCGG methylation in the banana genome. Plant Molecular Biology Reporter, 21 : pp. 339-348.

Journal article ; Article de revue à facteur d'impact
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Abstract : Two amplified fragment length polymorphism (AFLP)-derived techniques were used to assess methylation at CCGG sites in the banana genome. Assessment of these techniques revealed that, while amplification steps are very reproducible, the ligation step is more subject to variability. Overall, these techniques produced an error rate of 0.2% per analysed band. Statistical approach highlights the fact that sample duplication is necessary to produce reliable results. This study involved 18 primer pairs and found that in the banana genome, roughly 80% of CCGG sites are unmethylated, 5% are methylated at the internal cytosine, and 15% are methylated at the external or both cytosines. (Résumé d'auteur)

Mots-clés Agrovoc : Musa, Génome, ADN, Méthylation, Polymorphisme génétique, Marqueur génétique, PCR, Extraction, RFLP

Mots-clés complémentaires : AFLP

Classification Agris : F30 - Plant genetics and breeding

Auteurs et affiliations

  • Baurens Franc-Christophe, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0002-5219-8771
  • Bonnot François, CIRAD-CP-DIR (FRA)
  • Bienvenu David, CIRAD-AMIS-BIOTROP (FRA)
  • Causse Sandrine, CIRAD-FLHOR-PRH (FRA)
  • Legavre Thierry, CIRAD-AMIS-BIOTROP (FRA)

Autres liens de la publication

  • Document en bibliothèque
  • Localisation du document : CD_BR12405 [(Bibliothèque de Lavalette)] ; CD_BR12406 [(Bibliothèque de Lavalette)]

Source : Cirad - Agritrop (

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