Lipase-catalyzed synthesis of canola phytosterols oleate esters as cholesterol lowering argents

Villeneuve Pierre, Turon Fabrice, Caro Yanis, Escoffier R., Baréa Bruno, Barouh Nathalie, Lago R., Piombo Georges, Pina Michel. 2005. Lipase-catalyzed synthesis of canola phytosterols oleate esters as cholesterol lowering argents. Enzyme and Microbial Technology, 37 (1) : pp. 150-155.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.

Télécharger (284kB)

Abstract : The feasibility of lipase-catalyzed esterification of canola phytosterols with oleic acid was established. The influence of various reactions parameters was evaluated. Among the tested lipases, namely plant lipases from Carica papaya, Ricinus communis (castor bean lipase) or microbial lipases from Rhizomucor miehei, Candida antarctica B or Candida rugosa, the latter appeared to be the most appropriate resulting in a overall production of steryl ester of 38.5% within 24h in non-optimized conditions whereas esterification degree with other lipases did not exceed 15% in the same conditions. Selecting C. rugosa lipase for any further experiments, it was observed that the nature of the acyl donor was of paramount importance with direct esterification with fatty acids giving better results than transesterification with methyl esters. Similarly, the influence of substrates molar ratio was also checked and a 1:3 excess of fatty acids was shown to be the best. Finally, others parameters such as optimal enzyme load (5%) or temperature (35°C) were determined to allow a final production of steryl esters close to 85% after 72 h. (Résumé d'auteur)

Mots-clés Agrovoc : Estérase, Estérification, Huile de colza, Phytostérol, Acide oléique, Carica papaya, Ricinus communis, Biocatalyseur, Rhizomucor miehei, Candida rugosa, Pseudomonas cepacia, Activité catalytique, Cholestérol, Ester, Stérol, Lipase

Mots-clés complémentaires : Candida antarctica

Classification Agris : Q02 - Food processing and preservation
Q04 - Food composition

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

  • Villeneuve Pierre, CIRAD-AMIS-UMR IATE (FRA) ORCID: 0000-0003-1685-1494
  • Turon Fabrice, Bertin SA (FRA)
  • Escoffier R., CIRAD-AMIS-UMR IATE (FRA)
  • Baréa Bruno, CIRAD-AMIS-UMR IATE (FRA)
  • Barouh Nathalie, CIRAD-PERSYST-UMR IATE (FRA)
  • Piombo Georges, CIRAD-AMIS-UMR IATE (FRA)
  • Pina Michel, CIRAD-AMIS-UMR IATE (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-06-12 ]