Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa

Alberto Filipe, Correia L., Billot Claire, Duarte C.M., Serrao E.. 2003. Isolation and characterization of microsatellite markers for the seagrass Cymodocea nodosa. Molecular Ecology Notes, 3 (3) : 397-399.

Résumé : In order to study the spatial patterns of genetic diversity of a clonal marine angiosperm, the seagrass Cymodocea nodosa, microsatellite markers were obtained by screening a genomic library enriched for the (CT) dinucleotide motif. Of 38 primer pairs defined, 15 amplified polymorphic microsatellites and are described. These loci identified a number of alleles ranging from two to seven, and showed expected heterozygosity ranging from 0.35 to 0.76, when a group of 40 individuals from Cadiz Bay in Spain was analysed. Additionally, we describe here the multiplexing conditions for 12 of these loci.

Mots-clés Agrovoc : microsatellite, marqueur génétique, identification, algue marine

Mots-clés complémentaires : Cymodocea nodosa

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Alberto Filipe, Universidade do Algarve (PRT)
• Correia L., Universidade do Algarve (PRT)
• Billot Claire, CIRAD-AMIS-BIOTROP (FRA)
• Duarte C.M., Instituto mediterraneo de estudios avanzados (ESP)
• Serrao E., Universidade do Algarve (PRT)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/529434/)

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