Determination of polyethylene glycol 6000 (PEG) concentration in sheep faeces using near infrared spectroscopy

Hassoun Philippe, Bastianelli Denis, Bonnal Laurent. 2007. Determination of polyethylene glycol 6000 (PEG) concentration in sheep faeces using near infrared spectroscopy. In : Near infrared spectroscopy : Proceedings of the 12th International Conference, Auckland, New Zealand, 9th - 15th April 2005. Burling-Claridge G.R. (ed.), Holroyd S.E. (ed.), Sumner R.M.W. (ed.). NIRCE. Chichester : IM Publications, pp. 34-36. ISBN 978-0-473-11646-0|978-0-473-11746-7 International Conference on Near Infrared Spectroscopy. 12, Auckland, Nouvelle-Zélande, 9 April 2005/15 April 2005.

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Abstract : To assess dry matter intake (DMI) in grazing ruminants, techniques have been developed with chromium or ytterbium oxide as inert markers. These markers allow to estimate total faecal output quite accurately, but their analysis is costly and laborious so number of assays is generally limited. Some attempts to use Polyethylene Glycol (PEG, MW6000) as a faecal marker have already been mentioned in literature. NIRS estimation of PEG has been proposed because the reference method (turbidimetry) is tedious. A limitation for the use of NIRS is that faeces characteristics depend closely on the nature of the feed eaten by animals. Therefore calibrations based on a unique nature of faeces are not robust and fail to predict faeces PEG concentrations in animals fed with other diets. The aim of the present experiment is to develop models that predict PEG concentration in faecal output of sheep fed with various diets. A total of 585 samples was obtained by supplementation of 11 faeces with PEG added as a solution in the range 0 - 200 DM (by 5 or 10 step). Faeces samples originated from sheep flocks of several breeds and fed with different diets (total mixed diet, dehydrated diet, rangelands, improved pasture). Samples were ground (1 mm sieve) and diffuse reflectance spectra were taken in ring cups on a FOSS NIRSystem 6500. Calibration was carried out, using the modified partial least-squares method (MPLS, WINISI), with wavelengths in the 1100-2500 nm range after mathematical pre-processing (SNV and detrend, 2nd derivative). Equations developed with the whole concentration range (0-200 DM) on 545 samples (prepared from 10 different faeces) had R2=0.99 and SECV=4.3. However it was noticed that equations developed with narrower concentration ranges were more accurate. The SECV obtained with range scales from 0 to 60, 80, 100, 120 or 140 g PEG were 1.7, 2.3, 2.4, 2.8 and 3.2 respectively. The 11th series of samples (not used for calibration) was used for validation of the equations originating from whole and restricted scales. The standard error of prediction (SEP) was between 2.2-2.5 for the scales up to 100 g. kg-1 and between 4.0-4.6 over 100 In conclusion, NIRS is a good method to predict PEG content in sheep faeces. The precision levels obtained can support accurate prediction of DMI. The fact that the precision of equations is higher for smaller PEG concentrations is interesting since it allows to use lower PEG concentrations in experimental diets, which induces lower interactions with digestion phenomena. (Résumé d'auteur)

Mots-clés Agrovoc : Mouton, Fèces, Composition chimique, Polyéthylène

Classification Agris : U30 - Research methods
L50 - Animal physiology and biochemistry

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