Agritrop
Accueil

Identification of new gene expression regulators specifically expressed during plant seed maturation

Gutierrez Laurent, Conejero Geneviève, Castelain Mathieu, Guénin Stéphanie, Verdeil Jean-Luc, Thomasset Brigitte, Van Wuytswinkel Olivier. 2006. Identification of new gene expression regulators specifically expressed during plant seed maturation. Journal of Experimental Botany, 57 (9) : 1919-1932.

Article de revue ; Article de revue à facteur d'impact
[img] Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_533464.pdf

Télécharger (1MB)

Résumé : A cDNA-AFLP approach on Linum usitatissimum (flax) was used to identify genes specifically expressed during the seed maturation process. Among the 20 000 cDNA-AFLP tags produced, 486 were selected for their seed-specific expression during maturation. When compared with the publicly available databases, half of them presented some significant similarity with known plant sequences. The results obtained confirmed the accuracy of the approach as numerous genes previously described as being expressed exclusively in plant seeds were identified in this screen. The focus was on sequences similar to plant regulators involved in the control of gene expression, either at the transcriptional, post-transcriptional, or post-translational levels. Using a real-time RT-PCR approach, seed-specific expression kinetics were confirmed for 13 of these regulators that were never characterized for being expressed during seed maturation. Among these, a flax gene of the non-LEC1-like HAP3 family and a flax MYB factor were shown to be expressed in specialized tissues of flax embryo using an in situ hybridization approach. By expression kinetic comparison between these flax genes and their Arabidopsis counterparts, it was found that the new HAP3 gene should be related to a ubiquitous seed maturation mechanism, while a new MYB factor appears to be related to a more seed-specific maturation mechanism. These results demonstrate the utility of the flax database in not only identifying new genes expressed during seed maturation but also in being able to highlight the distinction between conserved and non-conserved seed maturation mechanisms.

Mots-clés Agrovoc : semence, maturation, banque de gènes, expression des gènes, identification, gène, adn, Transcription inverse, Linum usitatissimum, régulation physiologique, Arabidopsis, PCR

Mots-clés complémentaires : AFLP

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Gutierrez Laurent, Université de Picardie (FRA)
  • Conejero Geneviève
  • Castelain Mathieu, Université de Picardie (FRA)
  • Guénin Stéphanie, Université de Picardie (FRA)
  • Verdeil Jean-Luc, CIRAD-CP-UMR BEPC (FRA)
  • Thomasset Brigitte, Université de Compiègne (FRA)
  • Van Wuytswinkel Olivier, Université de Picardie (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/533464/)

Voir la notice (accès réservé à Agritrop) Voir la notice (accès réservé à Agritrop)

[ Page générée et mise en cache le 2024-01-28 ]