Genetic diversity and quinolone resistance in Campylobacter jejuni isolates from poultry in Senegal

Dieudonné Kinana Alfred, Cardinale Eric, Tall Fatou, Bahsoun Ibrahim, Sire Jean-Marie, Garin Benoit, Breurec Sébastien, Cheikh Saad-Bouth Boye, Perrier Gros-Claude Jean David. 2006. Genetic diversity and quinolone resistance in Campylobacter jejuni isolates from poultry in Senegal. Applied and Environmental Microbiology, 72 (5) : 3309-3313.

Résumé : We used the multilocus sequence typing (MLST) method to evaluate the genetic diversity of 46 Campylobacter jejuni isolates from chickens and to determine the link between quinolone resistance and sequence type (ST). There were a total of 16 ST genotypes, and the majority of them belonged to seven clonal complexes previously identified by using isolates from human disease. The ST-353 complex was the most common complex, whereas the ST-21, ST-42, ST-52, and ST-257 complexes were less well represented. The resistance phenotype varied for each ST, and the Thr-86-Ile substitution in the GyrA protein was the predominant mechanism of resistance to quinolone. Nine of the 14 isolates having the Thr-86-Ile substitution belonged to the ST-353 complex. MLST showed that the emergence of quinolone resistance is not related to the diffusion of a unique clone and that there is no link between ST genotype and quinolone resistance. Based on silent mutations, different variants of the gyrA gene were shown to exist for the same ST. These data provide useful information for understanding the epidemiology of C. jejuni in Senegal.

Classification Agris : L50 - Physiologie et biochimie animales

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Dieudonné Kinana Alfred, Institut Pasteur de Dakar (SEN)
• Cardinale Eric, CIRAD-EMVT-UPR Systèmes d'élevage (REU) ORCID: 0000-0002-3434-3541
• Tall Fatou, ISRA (SEN)
• Bahsoun Ibrahim, Institut Pasteur de Dakar (SEN)
• Sire Jean-Marie, Institut Pasteur de Dakar (SEN)
• Garin Benoit, Institut Pasteur de Dakar (SEN)
• Breurec Sébastien, Institut Pasteur de Dakar (SEN)
• Cheikh Saad-Bouth Boye, Hôpital Aristide Le Dantec (SEN)
• Perrier Gros-Claude Jean David, Institut Pasteur du Maroc (MAR)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/537711/)

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