Assessment of coffee berry disease using laboratory berry inoculation method

Pinard Fabrice, Omondi C.O.. 2007. Assessment of coffee berry disease using laboratory berry inoculation method. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, pp. 1380-1385. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 September 2006/15 September 2006.

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Abstract : This study was initiated to characterize the interaction between Colletotrichum kahawae and two coffee varieties at fruit level. Detached berries of Coffea arabica varieties, SL 28 (susceptible) and Ruim 11 (resistant) were artificially inoculated and kept under controlled conditions of 100% RH and a temperature of 17°C to 20°C. In a first experiment, the berries were inoculated with single spore culture of C. kahawae at various concentrations ranging from 2 * 106 sp/ml to 2 * 103 sp/ml. In a second experiment, berries of various sizes were inoculated with a single spore concentration. Statistical analysis (° 2, General Linear Model) of binary data (infected/healthy) and quantitative data (% of necrosis) revealed the existence of 2 resistance components operating against pathogen penetration and colonization of the berry tissue. The reaction was found to be controlled by at least 3 parameters: the inoculum concentration, the berry physiological stage and the host genotype. (Résumé d'auteur)

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding
U30 - Research methods

Auteurs et affiliations

  • Pinard Fabrice, CIRAD-CP-UPR Bioagresseurs de pérennes (KEN)
  • Omondi C.O., CRF (KEN)

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