The exploration of the pathosystem BSV/Musa sp. : How does it work?

Gayral Philippe, Lheureux Fabrice, Noa-Carrazana Juan Carlos, Lescot Magali, Piffanelli Pietro, Carreel Françoise, Jenny Christophe, Iskra Caruana Marie-Line. 2007. The exploration of the pathosystem BSV/Musa sp. : How does it work?. In : First Biosafenet Seminar, 6-8 June 2007, Venice, Italy. ICGEB. s.l. : s.n., Résumé, 1 p. Biosafenet Seminar. 1, Venise, Italie, 6 June 2007/8 June 2007.

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Abstract : As several other plants, the genome of banana and plantain contains integrations of Banana streak virus (BSV) sequences even though integration is not an essential step in the replication cycle of this virus. In banana two types of BSV integrants exist. Ones are non functional sequences present in both common Musa species, Musa acuminata (denoted A) and Musa balbisiana (denoted B) and it is now assumed that the integrants of the other type, containing the complete viral genome and restricted to M. balbisiana genome, become infectious by reconstituting a complete replication-competent viral genome. Thereby, an increasing record of BSV outbreaks was observed fifteen years ago among banana breeding lines and micro propagated inter-specific Musa hybrids, worldwide. Today, three widespread BSV species, Banana streak Obino l'Ewai virus (BSOIV), Banana streak Imové virus (BSImV) and Banana streak Golfinger virus (BSGfV) are known to occur as infectious integrants in the M. balbisiana genome. However, even though such integrations are known to be infectious, their presence is not sufficient to induce infection. We demonstrated that the process of genetic hybridization and abiotic stresses such as micropropagation by in vitro culture contributed in triggering episomal expression from EPRVs. Two mechanisms at least are involved in the BSV expression: the ploidy of the M. balbisiana in Musa genotypes and an additional genetic factor called BEL for BSV expressed locus concerning the triploids (Musa AAB) resulting from inter-species genetic crosses between virus-free diploid M. balbisiana (BB) and tetraploid M acuminata (AAAA) parents. Then, diploids M. balbisiana such as PKW and Pisang Batu harboring pathogenic BSV EPRVs are resistant to any multiplication of BSV while haploid genotypes such as triploids (AAB, French clair) or tetraploids (AAAB, FHIA 21) expressed BSV. Thereby, we characterized the segregation of three BSV species appearance among the AAB F1 progeny as a monogenic allelic system conferring the role of carrier to the M. balbisiana diploid parent. BSOIV and BSImV appeared in almost all infected hybrids (50% of the progeny) depending of BEL regulation while BSGfV are restricted in only half of these hybrids and subordinated by BEL. Three BAC libraries from accession of M.acuminata Cavendish subgroup cv petite naine (AAA), a wild M. acuminata subsp burmannicoides Calcutta 4 (AA) and a wild M. balbisiana PKW (BB) are explored for the pattern of integration of infectious BSV EPRVs by testing a set of different viral probes representing each time the BSOIV, lm and Gf complete genome. BSV positive BAC clones are characterised by RFLP fingerprints approaches. This analysis revealed that the three BSV species represent low-copy loci and their integration is specific to the PKW Musa balbisiana genome. BSGfV EPRVs in PKW is twice and are fully annotated after sequencing. Each BSGfV is composed of back-to-back viral sequences representing more than a whole BSV genome very similar each other. We developed molecular markers (PCR, PCR-RFLP) to distinguish each others and analysed the BSGfV EPRV segregation in the AAB F1 progeny. BSGfV EPRVs are found to be allelic, located at the same locus. In theory, both allelic EPRVs could be involved in the restitution of virions through a set of recombination events. (Texte intégral)

Mots-clés Agrovoc : Musa, Musa acuminata, Musa balbisiana, Virus des végétaux, Transmission des maladies, Relation hôte pathogène, Virologie, Séquence nucléotidique, Génome, Génie génétique

Mots-clés complémentaires : Banana streak virus

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Gayral Philippe, CIRAD-BIOS-UMR BGPI (FRA)
  • Lheureux Fabrice
  • Noa-Carrazana Juan Carlos
  • Lescot Magali
  • Piffanelli Pietro
  • Carreel Françoise
  • Jenny Christophe, CIRAD-BIOS-UPR Multiplication végétative (GLP)
  • Iskra Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449

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