Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis

Zouari Nabil, Ben Saad Rania, Legavre Thierry, Azaza Jalel, Sabau Xavier, Jaoua Mohamed, Masmoudi Khaled, Hassairi Afif. 2007. Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis. Gene, 404 : pp. 61-69.

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Abstract : Aeluropus littoralis (Gouan) Parl. is a C4 perennial halophyte monocotyledonous plant belonging to the same family of wheat. Growing as weed in dry salty areas or marshes, it is a salt-secreting, rhizomatous and is used as forage. It is diploid (2n=2X=14) and has a relative small genome of around 342 Mb. A. littoralis is highly salt-tolerant since this plant has the ability to secrete salt. Thus, A. littoralis has the potential to become an important genetic resource for biotechnological strategies to improve salt and drought tolerance in economically important crops such as wheat. We have constructed SSH (Suppression Subtractive Hybridization) cDNA libraries from root (RSD45) and leaf (LSD45) tissues of 45 days old plants grown in the presence of 300 mM NaC1. We have also constructed full-length cDNA library from 15 days old salt stressed (300 mM NaC1) roots (RSTL15). Sequencing revealed 25 and 42 independent transcripts from the RSD45 and LSD45 cDNA libraries respectively, in both cases this was less than 25% of the clones sequenced. In contrast, 425 (60%) of the clones from the RSTL15 library revealed independent transcripts. After comparison with protein databases using BlastX, 335 (68%) ESTs (Expressed Sequence Tag) were classified into putative known functions and unclassified proteins, 59 (12%) have homology only to unidentified homologous sequences. A total of 98 (20%) of the ESTs have no homologies to known sequences in the protein databases which can be considered as novel. © 2007 Elsevier B.V. All rights reserved.

Mots-clés Agrovoc : Poaceae, Identification, ADN, Stress osmotique, Génie génétique, Amélioration des plantes, Collection de matériel génétique, Tolérance au sel, Hybridation d'ADN, Triticum aestivum

Mots-clés complémentaires : Aeluropus littoralis, Séquencage

Classification Agris : F30 - Plant genetics and breeding
H50 - Miscellaneous plant disorders

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

  • Zouari Nabil, Centre de biotechnologie (TUN)
  • Ben Saad Rania, Centre de biotechnologie (TUN)
  • Legavre Thierry, CIRAD-BIOS-UMR DAP (FRA)
  • Azaza Jalel, Centre de biotechnologie (TUN)
  • Sabau Xavier, CIRAD-BIOS-UMR DAP (FRA)
  • Jaoua Mohamed, Centre de biotechnologie (TUN)
  • Masmoudi Khaled, Centre de biotechnologie (TUN)
  • Hassairi Afif, Centre de biotechnologie (TUN)

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