Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant

Garzon Edwin, Bossard Géraldine, Bras R., Totté Philippe, Rodrigues Valérie, Boulanger A., Authié Edith, Dedieu Laurence. 2007. Trypanosoma congolense heat-shock protein 70 : a promising TH1 adjuvant. In : Does control of animal infectious risks offer a new international perspective ? : proceedings of the 12th International Conference of the Association of Institutions of Tropical Veterinary Medicine, Montpellier, France, 20-22 August 2007. Camus Emmanuel (ed.), Cardinale Eric (ed.), Dalibard Christophe (ed.), Martinez Dominique (ed.), Renard Jean-François (ed.), Roger François (ed.). CIRAD. Montpellier : CIRAD, 201-204. ISBN 978-2-87614-650-1 International Conference of the Association of Institutions of Tropical Veterinary Medicine. 12, Montpellier, France, 20 Août 2007/22 Août 2007.

Résumé : Developing immunopotentiators to improve vaccine efficacy in ruminants is an important goal. Heat shock proteins (HSPs) have been shown to act as adjuvant when co-administrated with peptides antigens or given as fusion proteins. In this study, we have investigated the role of Trypanosoma congolense HSP-70 (Tcong-HSP-70) on the maturation of murine dendritic cells (DCs) generated from bone marrow precursor cells. The results obtained showed that Tcong-HSP-70 induced murine DC maturation as determined by increased levels of surface markers (CD40, CD86 and MHC II) as well as proliferation of allogeneic splenocytes. DCs exposed to Tcong-HSP-70 were characterized by strong expression of TNF[alpha] and IL-12p70 and moderate expression of IL-10. Similar study, now underway, on bovine monocyte-derived DCs demonstrated the ability of Tcong-HSP-70 to also trigger bovine Mo-DC maturation. This finding thus suggests that Tcong-HSP-70 can act as a potent Th1 adjuvant for antigen delivery.

Classification Agris : L72 - Organismes nuisibles des animaux

Auteurs et affiliations

• Garzon Edwin
• Bossard Géraldine, CIRAD-BIOS-UMR TRYPANOSOMES (FRA)
• Bras R., IRD (FRA)
• Totté Philippe, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Rodrigues Valérie, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Boulanger A.
• Authié Edith, INRA (FRA)
• Dedieu Laurence, CIRAD-BIOS-UPR Contrôle des maladies (FRA) ORCID: 0000-0002-6492-3002

Source : Cirad - Agritrop (https://agritrop.cirad.fr/541572/)

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