Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia

Totté Philippe, Rodrigues Valérie, YaYa Aboubakar, Hamadou B., Cissé Ousmane, Diallo Mahamadou, Niang Mamadou, Thiaucourt François, Dedieu Laurence. 2008. Analysis of cellular responses to Mycoplasma mycoides subsp. mycoides small colony biotype associated with control of contagious bovine pleuropneumonia. Veterinary Research, 39 (1) : 1-11.

Résumé : A better understanding of protective immune memory against contagious bovine pleuropneumonia (CBPP) is needed in order to facilitate the development of safer vaccines based on selected components of the pathogen. For this purpose, cells collected from lymph nodes draining the lungs of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC)-infected cattle were stimulated with the pathogen in vitro and evaluated concurrently for proliferation (CFSE based method), expression of activation, memory markers and cytokine production. Direct evidence is presented for a major contribution of CD4+T cells to the vigorous proliferative and T1 biased cytokine recall responses observed in cattle that have recovered from infection but not in animals developing the acute form of the disease. Two different phenotypes of MmmSC-specific memory CD4 were observed based on CD62L expression and proliferative capacities. Furthermore, recall proliferation of B cells also occurred but was strictly dependent on the presence of CD4. The information provided in this study will facilitate the search for MmmSC antigens that have potential for the development of subunit vaccines against CBPP.

Mots-clés Agrovoc : Mycoplasma mycoides, péripneumonie contagieuse bovine, vaccin, lymphocyte, immunologie

Mots-clés complémentaires : Lymphocyte T

Classification Agris : L73 - Maladies des animaux
L72 - Organismes nuisibles des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Totté Philippe, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Rodrigues Valérie, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• YaYa Aboubakar, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Hamadou B., LANAVET (CMR)
• Cissé Ousmane, LCV (MLI)
• Diallo Mahamadou, LCV (MLI)
• Niang Mamadou, LCV (MLI)
• Thiaucourt François, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
• Dedieu Laurence, CIRAD-BIOS-UPR Contrôle des maladies (FRA) ORCID: 0000-0002-6492-3002

Source : Cirad - Agritrop (https://agritrop.cirad.fr/543267/)

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