A large EST resource for theobroma cacao L.

Lanaud Claire, Fouet Olivier, Gramacho Karina Peres, Argout Xavier, Legavre Thierry, Sabau Xavier, Risterucci Ange-Marie, Wincker Patrick, Da Silva Corinne, Loor Solorzano Rey Gaston, Cascardo Julio Cezar M., Courtois Brigitte, Kuhn David N., Schnell Rey J., Babin Régis, Sounigo Olivier, Ducamp Michel, Deberdt Peninna, Verica Joseph, Guiltinan Mark J., Alemanno Laurence, Machado Regina, Philips Wilbert, Maximova Siela N., Rosenquist Eric, Gilmour Martin, Glaszmann Jean-Christophe. 2007. A large EST resource for theobroma cacao L.. In : Abstracts of Plant and Animal Genomes XVth Conference, San Diego, CA (USA), January 13-17, 2007. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 15, San Diego, États-Unis, 13 January 2007/17 January 2007.

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Abstract : An international project aiming to sequence a large collection of 180,000 cocoa cDNA enriched in full length cDNAs was carried out. CDNA were isolated from different organs, sometimes submitted to various biotic and abiotic stresses. About 45 cDNA libraries were constructed from a wide panel of organs: flowers, flower cushions, seeds at different stages of development and during fermentation, cherels, pod cortex, shoot, wood, root, germinated seeds, and embryos from vitro culture. Direct and SSH libraries were constructed from organs subjected to biotic or abiotic stresses: leaves and pods inoculated with Phytophthora palmivora and Phytophthora megakarya, shoots and/or pods inoculated with Crinipellis perniciosa, Moniliophthora roreri, Ceratocystis fimbriata, and shoots attacked by mirids. leaves and roots under drought conditions. The construction and management of cDNA libraries, was performed with the help of the robotic platform, part of the "Montpellier Languedoc-Roussillon GENOPOLE". All the sequencing work was performed by GENOSCOPE (from the GENOPOLE Evry/France). A bioinformatic pipeline (ESTtik) was constructed; it automatically processes the sequences, assembles, annotates them, and integrates the results into a web-based database, allowing researchers browse and query of the results. About 62% of cocoa sequences showed significant similarity to gene sequences from other species and a part of them could be classified according to the Gene Ontology. This new molecular cocoa resource allowed the identification of hundreds of new microsatellite markers and thousands of SNPs (Single Nucleotide Polymorphisms). It will allow to identify an unigene set suitable for all functional genomic studies in cocoa. (Texte intégral)

Mots-clés Agrovoc : Theobroma cacao, Séquence nucléotidique, ADN, Agent pathogène, Collection de matériel génétique

Mots-clés géographiques Agrovoc : France

Classification Agris : F30 - Plant genetics and breeding
H01 - Protection of plants - General aspects
F70 - Plant taxonomy and geography

Auteurs et affiliations

  • Lanaud Claire, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-6411-7310
  • Fouet Olivier, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-8547-1474
  • Gramacho Karina Peres, CEPEC (BRA)
  • Argout Xavier, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0002-0100-5511
  • Legavre Thierry, CIRAD-BIOS-UMR DAP (FRA)
  • Sabau Xavier, CIRAD-BIOS-UMR DAP (FRA)
  • Risterucci Ange-Marie, CIRAD-BIOS-UMR DAP (FRA)
  • Wincker Patrick, Centre national de séquençage (FRA)
  • Da Silva Corinne, Centre national de séquençage (FRA)
  • Loor Solorzano Rey Gaston, CIRAD-BIOS-UMR DAP (FRA)
  • Cascardo Julio Cezar M., UESC (BRA)
  • Courtois Brigitte, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0003-2118-7102
  • Kuhn David N., USDA (USA)
  • Schnell Rey J., USDA (USA)
  • Babin Régis, CIRAD-BIOS-UPR Bioagresseurs de pérennes (CMR) ORCID: 0000-0002-3753-1193
  • Sounigo Olivier, CIRAD-BIOS-UPR Bioagresseurs de pérennes (CMR) ORCID: 0000-0001-6278-8903
  • Ducamp Michel, CIRAD-BIOS-UMR BGPI (FRA)
  • Deberdt Peninna, CIRAD-BIOS-UPR Bioagresseurs de pérennes (TTO)
  • Verica Joseph, Pennsylvania State University (USA)
  • Guiltinan Mark J., Pennsylvania State University (USA)
  • Alemanno Laurence, CIRAD-CP-UMR BEPC (FRA)
  • Machado Regina, Masterfoods (BRA)
  • Philips Wilbert, CATIE (CRI)
  • Maximova Siela N., Pennsylvania State University (USA)
  • Rosenquist Eric, USDA (USA)
  • Gilmour Martin, MARS Confectionery (GBR)
  • Glaszmann Jean-Christophe, CIRAD-BIOS-DIR (FRA) ORCID: 0000-0001-9918-875X

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