Characterization of the model system rice Magnaporthe for the study of nonhost resistance in cereals

Faivre-Rampant Odile, Thomas Justine, Allègre Mathilde, Morel Jean-Benoit, Tharreau Didier, Nottéghem Jean-Loup, Lebrun Marc-Henri, Schaffrath Ulrich, Piffanelli Pietro. 2008. Characterization of the model system rice Magnaporthe for the study of nonhost resistance in cereals. New Phytologist, 180 (4) : 899-910.

https://doi.org/10.1111/j.1469-8137.2008.02621.x

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Résumé : The best characterized form of resistance is gene-for-gene resistance. Less well characterized is nonhost resistance in which an entire plant species is resistant to an entire pathogen species. Here, different rice genotypes were inoculated with host and nonhost strains of Magnaporthe isolated from rice, wheat and crabgrass.The different types of interactions were characterized at a cytological level using a 3,3'-diaminobenzidine (DAB) stain to investigate the occurrence of reactive oxygen intermediates or by observing the occurrence of cellular autofluorescence. Gene expression of a set of selected PR-genes was analysed using quantitative real-time polymerase chain reaction.Inoculation with the isolate from crabgrass resulted in a lack of penetration. The wheat isolate induced a hypersensitive response with varying degrees of pathogen growth inside the invaded cell according to the rice genotype. Expression analysis of our PR-gene set revealed clear differences between the different types of interactions in both kinetic and magnitude of gene induction.Our integrated study opens the way to the dissection of molecular components leading to nonhost reactions to Magnaporthe grisea in rice and points to novel sources of durable resistance to fungal plant pathogens in other cereal crops.

Mots-clés Agrovoc : Oryza sativa, Magnaporthe, interaction génique, résistance aux maladies, mécanisme de défense, génétique moléculaire, plante hôte, céréale

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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